Nucleic acid detection kit for detecting BRCA1mRNA (breast cancel susceptibility gene1 messenger ribonucleic acid)

A detection kit and nucleic acid technology, applied in the fields of life science and biology, can solve the problems of high cost and inferior specificity, and achieve the effect of reducing detection cost, simple operation and saving detection time.
CN103451282AActive Publication Date: 2013-12-18杭州艾迪康医学检验中心有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
杭州艾迪康医学检验中心有限公司
Publication Date
2013-12-18

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Abstract

The invention discloses a nucleic acid detection kit for detecting BRCA1mRNA (breast cancel susceptibility gene1 messenger ribonucleic acid). The nucleic acid detection kit comprises red blood cell lysis buffer, RNA extracting solution, detection system PCR (polymerase chain reaction) liquid, a positive reference substance and a negative reference substance. The nucleic acid detection kit is characterized in that the detection system PCR liquid comprises PCR buffer solution, dNTP (diethyl-nitrophenyl thiophosphate), Mg<2+>, upstream and downstream primers BRCA1-F / BRCA1-R and a probe BRCA1-Probe for detection, upstream and downstream primers Actin-F / Actin-R probe Actin-Probe for reference, in which, BRCA1-F:CAGCTACCCTTCCATCATAAGTGA; BRCA1-R:GGCCATGTATATGCGAATCTTTTT; BRCA1-Probe:FAM-TTCTGCCCTTGAGGACCTGCGAAAT-TAMRA; Actin-F:TGAGCGAGGCTACAGCTT; Actin-R:TCCTTGATGTCGCGCACGATTT; Actin-Probe:FAM-ACCACCACGGCCGAGCGG-TAMRA.
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Description

technical field

[0001] The invention belongs to the field of life science and biotechnology, in particular to a gene detection kit, which can detect the expression level of BRCA1 in human breast cancer by using probe real-time fluorescent quantitative PCR technology, which can effectively save detection time and improve detection precision. Background technique

[0002] Breast cancer is a common tumor in women and has become the second leading cause of cancer death. Its diagnosis is mainly based on imaging examinations, lacking laboratory-specific diagnostic indicators, which brings difficulties to early diagnosis and is easy to be missed or misdiagnosed. Surgical resection is the first choice for the treatment of breast cancer, but the shape is affected after surgery, and the appearance is not good. It has caused great distress to patients, and some patients have psychological shadows caused by it, which seriously affects their quality of life. At present, it is generally...

Claims

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