Hot flash suppressant

A technology for suppressing hot flashes, applied in the field of suppressing hot flashes, can solve unclear problems

Inactive Publication Date: 2013-12-18
SHISEIDO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, it is not completely clear that apelin is related to the suppression and improvement of hot flashes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1: The hot flash inhibitory effect of apelin peptide

[0104] Under the control of the K14 promoter, the C57 / BL6 mice (hereinafter referred to as apelin high-expressing mice, apelin TG, or TG) that make apelin highly expressed in the epidermis and corresponding wild animals Type mice (C57 / BL6) (N=5, all 8-10 weeks old) underwent ovariectomy to produce climacteric apelin high-expressing mice and climacteric wild-type mice. In addition, wild-type mice subjected to sham surgery were used as controls. Four weeks later, nifedipine (50 μg / kg) as a vasodilator was intraperitoneally administered, and after 10 minutes, 30 minutes, and 45 minutes after the administration, the temperature recording method (Somatoray SA TH9100MR, NEC / Avio infrared technology) to analyze temperature changes in the tail.

[0105] result

[0106] For the wild-type mice (shamWT) of the control, the skin temperature that rose after 10 minutes of vasodilator administration returned to a s...

Embodiment 2

[0107] Example 2. Establishment of a screening system for apelin-like agents

[0108] Culture of APJ expressing cells

[0109] In NIH3T3 cells, the apelin receptor APJ was highly expressed, and the cAMP-luciferase reporter vector was transfected in a stable strain selected by hygromycin (provided by Professor Takakura Nobuyuki, Institute of Microbial Diseases, Osaka University). It was used in the cAMP luciferase assay below. The culture was carried out using DMEM medium (Invirtogen) containing 200 μg / ml hygromycin and 10% FBS. On the day before the assay, inoculate 2.0 × 10 per well in a 96-well plate 5 cells were cultured in DMEM medium containing 10% FBS and 200 μg / ml hygromycin.

[0110] cAMP luciferase assay

[0111] After culturing overnight, the medium was removed with a pipette, and replaced with 100 µl per well of DMEM medium without additives. After 3 hours, the GloSensor TM 4 μl of cAMP reagent (Promega) was added to each well, shielded from light, and equi...

Embodiment 3

[0113] Example 3. Screening of apelin peptide-like agents (1)

[0114] As candidate drugs, about 250 kinds of cosmetic raw materials (Shiseido Co., Ltd.) were used to select an apelin-like drug through the screening system established in Example 2.

[0115] Specifically, the candidate drug was adjusted to 400 μg / ml, and 50 μl (final concentration: 100 μg / ml) was added to each well instead of the apelin peptide used in the cAMP luciferase assay of Example 2, and the same procedure as in Example 2 was carried out. 2 for the same determination.

[0116] Such as Figure 4 As indicated, saffron extract, pine tree extract, cinchona extract and comfrey extract, scutellaria baicalensis extract, rosemary extract, almond grains, gentian extract, thyme extract, peppermint powder, jujube extract extract, hops extract, kiwi fruit extract, fruity chrysanthemum extract, apple extract, hawthorn extract and turmeric extract, the intracellular cAMP concentration was significantly reduced, a...

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Abstract

The present invention provides: a hot flash suppressant which contains apelin or an apelin receptor agonist; the hot flash suppressant wherein the apelin receptor agonist is selected from the group consisting of Rubus buergeri, Kalimeris yomena, Stauntonia hexaphylla, Rubus grayanus, Kalimeris indica, Pasania edulis, Merica rubra, Syzygium jambos, Quercus serrata and extracts of these plants, as well as a saffron extract, a pine extract, a cinchona extract, a comfrey extract, a Scutellaria extract, a rosemary extract, an apricot seed powder, a gentian extract, a thyme extract, a mint powder, a Zizyphi fructus extract, a hop extract, a kiwifruit extract, a roman chamomile extract, an apple extract, a hawthorn extract and a turmeric extract; and a screening method for a medical agent for improving hot flash.

Description

technical field [0001] The present invention relates to an inhibitor of hot flashes caused by menopausal disorders and a method for improving hot flashes. Background technique [0002] Hot flashes (a localized rise in body surface temperature, accompanied by dizziness, fever, flushing, and hyperhidrosis), which are one of the menopausal disorders, are general symptoms of menopausal / postmenopausal syndrome. The decline of female hormones (estrogen, etc.) is considered to be the cause of menopausal disorders represented by hot flashes, and in young women, hormone balance may collapse due to excessive stress, diet, etc., and symptoms similar to menopausal disorders may appear. As a treatment method for these climacteric disorders and symptoms similar to climacteric disorders, estrogen supplementation may be performed. However, estrogen supplementation therapy is known to have side effects such as an increased risk of breast cancer, endometrial cancer, etc., and long-term estro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/00A61K45/00A61P15/12C12Q1/02C12Q1/68G01N33/15G01N33/50C07K14/47C12N15/09
CPCA61K36/00A61K38/00A61K38/1709A61K39/395A61K45/06A61P15/12A61P43/00C07K14/47G01N2333/726A61K2300/00
Inventor 泽根美加加治屋健太朗高木雅哉
Owner SHISEIDO CO LTD
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