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Preparation method and application of microalgae peptide skin agent resisting bacterium and pruritus and reducing inflammation and swelling

A technology of detumescence agent and microalgae, which is applied in the field of biomedicine, can solve the problems of no literature reports, etc., and achieve the effects of stable drug effect, improved utilization rate, and significant social and economic benefits

Active Publication Date: 2013-12-25
FUJIAN LANHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no literature report on the application of microalgae meal enzymolysis peptide to treat skin diseases

Method used

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  • Preparation method and application of microalgae peptide skin agent resisting bacterium and pruritus and reducing inflammation and swelling
  • Preparation method and application of microalgae peptide skin agent resisting bacterium and pruritus and reducing inflammation and swelling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Preparation of microalgae peptide skin antibacterial, antipruritic, anti-inflammatory and anti-swelling agent

[0027] Schizochytrium algae DHA (docosahexaenoic acid) oil scumbag meal, crushed into powder without particles, weighed 1 kg, placed in an enzymatic hydrolysis tank, added 8 L of PBS with a pH of 7.2, and stirred evenly Finally, add 10 g pepsin and 10 g trypsin, hydrolyze at 37 ° C for 3 h, cool to room temperature, centrifuge at 4500 rpm, and centrifuge for 10 min to separate the supernatant and precipitate, and take the supernatant; Under the conditions of ℃ temperature and vacuum degree of 0.09 MPa, vacuumize for 1 hour to inactivate the enzyme and remove the fishy smell; use ultrafiltration membrane ultrafiltration with a molecular weight cut-off of 6000Da, take the ultrafiltration liquid, and freeze-dry to obtain microalgae peptide skin antibacterial Antipruritic anti-inflammatory detumescence agent.

[0028] (2) Antibacterial experiment

[0...

Embodiment 2

[0037] Scutellaria chinensis DHA (docosahexaenoic acid) oil scumbag meal, crushed into powder without granules, weighed 1 kg, placed in an enzymatic hydrolysis tank, added 10 L of PBS with a pH of 7.4, and stirred evenly Finally, add 20 g pepsin and 20 g trypsin, hydrolyze at 37 °C for 6 h, cool to room temperature, centrifuge at 4500 rpm, and centrifuge for 10 min to separate the supernatant and precipitate, and take the supernatant; Under the conditions of ℃ temperature and vacuum degree of 0.09 MPa, vacuumize for 1.5 h to inactivate the enzyme and remove the fishy smell; use ultrafiltration membrane ultrafiltration with a molecular weight cut-off of 5000Da, take the ultrafiltration liquid, and freeze-dry to obtain microalgae peptide skin antibacterial Antipruritic anti-inflammatory detumescence agent.

[0038] The microalgae peptide skin antibacterial, antipruritic, anti-inflammatory and anti-swelling agent has been tested on human skin irritation, and no erythema, papule, ...

Embodiment 3

[0040]Cryptidinium koushii DHA (docosahexaenoic acid) oil scumbag meal, crushed into powder without granules, weighed 1 kg, placed in an enzymatic hydrolysis tank, added 6 L of PBS with a pH of 7.4, and stirred evenly Finally, add 10 g pepsin and 10 g trypsin, hydrolyze at 37 ℃ for 5 h, cool to room temperature, centrifuge at 6000 rpm, and centrifuge for 20 min to separate the supernatant and precipitate, and take the supernatant; Under the conditions of ℃ temperature and vacuum degree of 0.10 MPa, vacuumize for 2 hours to inactivate the enzyme and remove the fishy smell; use the ultrafiltration membrane ultrafiltration with a molecular weight cut-off of 4000Da, take the ultrafiltration liquid, freeze-dry, and obtain the microalgae peptide skin antibacterial Antipruritic anti-inflammatory detumescence agent.

[0041] The microalgae peptide skin antibacterial, antipruritic, anti-inflammatory and anti-swelling agent has been tested on human skin irritation, and no erythema, papu...

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Abstract

The invention discloses a preparation method and application of a microalgae peptide skin agent resisting bacterium and pruritus and reducing inflammation and swelling. Specifically, docosahexenoic acid is prepared by microalgae, namely, the leftover bits and pieces of DHA (docosahexenoic acid) algae dregs are taken as a raw material; the microalgae peptide skin agent resisting bacterium and pruritus and reducing inflammation and swelling is prepared through steps of smash, enzymolysis, centrifugation, heating in water bath, vacuumizing, ultrafiltration, and freeze drying. The prepared skin powder agent for external use has effects of resisting bacterium and pruritus and reducing inflammation and swelling, and is applicable to local treatment of neurodermatitis, contact dermatitis, chronic eczema, insect bite dermatitis and pruritus skin disease. The preparation method is innocuous and unpoisonous, is free from pollution, is excellent and stable and reliable, and turns the waste algae dregs into medicine products, thus greatly improving the use ratio of the waste, and having remarkable social and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and more specifically relates to a preparation method and application of a microalgae peptide skin antibacterial, antipruritic, anti-inflammatory and anti-swelling agent. Background technique [0002] Skin itching, redness, and inflammation are common skin diseases and one of the frequently-occurring diseases that seriously affect people's health. They are usually divided into neurodermatitis, contact dermatitis, chronic eczema, insect bite dermatitis, and itchy skin diseases. . Patients usually feel itching, pain, numbness, burning, etc. and cannot help scratching or rubbing, which can lead to skin scars and, in severe cases, skin cancer. Therefore, a non-toxic, non-hazardous and side-effect-free drug with antibacterial, antipruritic, anti-inflammatory and anti-swelling effects on the skin is urgently desired by many patients, and the biopeptide after protein degradation of microalgae meal ...

Claims

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Application Information

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IPC IPC(8): A61K36/06A61K36/02A61P17/00A61P17/04A61P31/04
Inventor 李昊
Owner FUJIAN LANHAO BIOTECH
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