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Cordyceps sinensis medium for large-scale liquid submerged fermentation and fermentation production method thereof

A liquid submerged fermentation and Cordyceps sinensis technology, applied in the direction of fungi, etc., can solve the problems of low mycelium yield, long fermentation cycle, and large energy consumption, and achieve increased mycelium production, short fermentation cycle, and energy consumption. little effect

Inactive Publication Date: 2015-08-05
东方中科生命科学有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some domestic scientific research workers have published some related patents and papers, disclosing some culture media and cultivation techniques for liquid fermentation of Cordyceps sinensis, but there are generally long fermentation cycles, easy pollution, cumbersome steps, large energy consumption, and low mycelium productivity. high problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Mother seed preparation

[0022] The weight ratio of each component of the solid medium prepared by the mother species is: 3% carbon source (the carbon content is obtained by calculating the molecular weight), the carbon source is glucose or sucrose or maltose, 2% nitrogen source (the nitrogen content is obtained by measurement), the nitrogen source It is yeast extract or beef extract or peptone; 0.1% magnesium sulfate, 0.15% potassium dihydrogen phosphate, 0.08% multivitamin, 1.00% agar powder, and the rest is water. The multivitamin is composed of vitamin B1, vitamin B2 and riboflavin, and the weight ratio is 1:1:1 or 2:2:1;

[0023] Mix the above materials, heat to 100°C, dissolve and mix the materials, sterilize at 121°C under 0.15MPa pressure for 40 minutes, cool to room temperature and solidify.

[0024] Fresh Cordyceps sinensis (Latin scientific name Cordyceps sinensis, also written as Ophiocordyceps sinensis) is harvested in the Cordyceps sinensis productio...

Embodiment 2

[0038] In embodiment 1, increase the primary seed tank of 5L, 50L and 500L are as secondary and tertiary seed tank, all the other technical processes, culture conditions are all exactly the same as embodiment 1, obtain pure Cordyceps sinensis dry bacterium powder, yield 9.2%.

Embodiment 3

[0040] Compared with Example 1, the seed tank culture inoculum ratio increased to 5%, the fermenter culture inoculum ratio increased to 10%, and all the other technological processes and culture conditions were exactly the same as in Example 2 to obtain pure dry bacterium powder of Cordyceps sinensis , the yield is 11%.

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Abstract

The invention discloses a culture medium for large-scale liquid submerged fermentation of Cordyceps sinensis and a fermentation production method thereof. The mother species is prepared from Cordyceps sinensis harvested from the Qinghai-Tibet Plateau, and the mother species is put into a triangular flask for shaker culture to prepare a liquid strain. Then it is connected to seed tanks of all levels for fermentation and cultivation, and it is enlarged step by step. Finally, large-scale liquid submerged fermentation of Cordyceps sinensis is realized in the fermentation tank. The final volume of the fermentation tank reaches 5 tons or more, and the dry weight of mycelium reaches 5-15 tons. %. The invention adopts high-quality Cordyceps sinensis strains, a special culture medium and a special fermentation process, and realizes the purposes of promoting the growth of the Cordyceps fungus, shortening the construction period, reducing pollution opportunities, saving energy and reducing costs.

Description

technical field [0001] The invention belongs to the technical field of fermentation production of edible and medicinal fungi, and in particular relates to a culture medium for large-scale liquid submerged fermentation of Cordyceps sinensis and a fermentation production method thereof. Background technique [0002] Cordyceps sinensis belongs to the fungus species of Ergotaceae (the Latin scientific name is Cordyceps sinensis, also written as Ophiocordyceps sinensis), also known as Chinese hairy spores or Chinese hairy hairs. Cordyceps sinensis has a long history of application in Chinese folk, and it is a high-grade and precious Chinese herbal tonic. Its health care and medical effects are recorded in "Materia Medica Congxin" and "Pharmacopoeia of the People's Republic of China". Cordyceps sinensis is distributed in Qinghai, Tibet and other high-altitude cold areas of the Qinghai-Tibet Plateau, and is mainly produced in the upper reaches of the Jinsha River, Lancang River, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 李文新陈春森廖金永
Owner 东方中科生命科学有限责任公司
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