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A kind of preparation method of splinter Ulva sulfate polysaccharide with antitumor activity

A technology of splinter Ulva and sulfated polysaccharide is applied in the directions of antitumor drugs, organic active ingredients, medical preparations containing active ingredients, etc. The effect of reducing process cost, suitable for industrial production and simple equipment

Active Publication Date: 2016-02-24
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using chemical methods to separate and purify polysaccharides often has severe reaction conditions, which will lead to changes in the conformation of polysaccharides, easy damage to active groups, and unstable product quality, which will seriously affect the biological activity of polysaccharides.
[0006] The publication number is CN200610026830.7 which discloses the application of a centipede algae polysaccharide extract in the preparation of anti-tumor and other drugs, but this method only involves the extraction of the centipede algae crude polysaccharide without further purification and separation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Wash Ulva lobula, dry at 60°C, and pulverize to obtain a powder, and pass through a 100-mesh sieve to obtain a pulverized product with uniform particles.

[0029] (2) Weigh 60g of the pulverized material in step (1), add 3600ml of distilled water with a pH of 7.0 at a material-to-liquid ratio of 1:60, and conduct ultrasonic-assisted extraction with ultrasonic power 480W for 30 minutes. Cool and stand for 1 hour, then centrifuge at 10,000 r / min and 4°C for 10 minutes to obtain supernatant and precipitate.

[0030] (3) Concentrate the supernatant obtained in step (2) by rotary evaporation until the volume is 1 / 10 of the original volume, add an ethanol solution with a volume fraction of 95% so that the final volume concentration of ethanol is 80%, place it for 15 hours, 1000r / min, centrifuged at 4°C for 10 min, and the resulting precipitate was freeze-dried to obtain 12.0 g of crude polysaccharide at the ethanol concentration.

[0031] (4) Weigh the crude polysacchar...

Embodiment 2

[0037] (1) Wash Ulva lobula, dry at 60°C, and pulverize to obtain a powder, and pass through a 100-mesh sieve to obtain a pulverized product with uniform particles.

[0038] (2) Weigh 60g of the pulverized material in step (1), add 4200ml of distilled water with a pH of 7.0 at a material-to-liquid ratio of 1:70, and conduct ultrasonic-assisted extraction with ultrasonic power of 500W for 40min. Cool and stand for 1 hour, then centrifuge at 10,000 r / min and 4°C for 10 minutes to obtain supernatant and precipitate.

[0039] (3) Concentrate the supernatant obtained in step (2) by rotary evaporation until the volume is 1 / 10 of the original volume, add an ethanol solution with a volume fraction of 95% so that the final volume concentration of ethanol is 80%, leave it for 20 hours, 1000r / min, centrifuged at 4°C for 10 min, and the resulting precipitate was freeze-dried to obtain 12.8 g of crude polysaccharide at the ethanol concentration.

[0040] (4) Weigh the crude polysaccharid...

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PUM

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Abstract

The invention discloses a preparation method of an ulva fasciata delile sulfated polysaccharide with anti-tumor activity. The preparation method comprises the following steps of crushing and ultrasonically extracting ulva fasciata delile by water to prepare a crude polysaccharide through alcohol precipitation; carrying out depigmentation deproteinization by using a radial-flow chromatographic column which is filled with weakly basic anion exchange resin; carrying out ultrafiltration separation according to molecular weight after elution; and taking and adding an ulva fasciata delile polysaccharide compound with molecular weight of 10 KD to 30 KD into the radial-flow chromatographic column which is filled with DEAE (Diethyl Aminoethanol) sepharose gel for enriching and purifying, wherein the prepared polysaccharide with average molecular weight of 20 KD has remarkable antitumor activity.

Description

technical field [0001] The invention relates to the research and development of marine algae polysaccharides, in particular to a preparation method and application of Ulva sliver sulfate polysaccharides with anti-tumor activity, belonging to the technical field of marine biology. Background technique [0002] The ocean area is vast, and the sources of marine algae are abundant. Seaweed polysaccharides are gradually becoming one of the main sources of biological polysaccharides. Seaweed polysaccharides have high medicinal value, and have significant effects in improving human non-specific immune function, anti-oxidation, anti-tumor, anti-radiation, anti-aging, lowering blood sugar and blood fat. The active substances in seaweed polysaccharides are mostly water-soluble acidic and neutral polysaccharides composed of monosaccharides such as D-glucose, D-mannose, D-galactose, and D-glucuronic acid. [0003] At present, different methods are used for polysaccharide extraction, in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/737A61P35/00
Inventor 邵平秦敏朴孙培龙
Owner ZHEJIANG UNIV OF TECH