Phomopsis sp. fungus strain and application to fungus-line wood preparation thereof

A technology of Phomopsis and fungus, applied in the field of microorganisms, can solve the problems of decay or discoloration, increased cost, and inability to process and use, and achieve good infection effect and eliminate dead ends of treatment

Inactive Publication Date: 2014-01-08
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the naturally obtained fungal wood still has the following deficiencies: 1. The raw materials are not easy to obtain, and there is a strong chance, because the natural fungal wood can only be formed in nature when there are bacteria that can form the fungal wood. , so only a small amount of fungal wood can be found in the dead wood in a small part of the forest; 2. The natural fungal wood takes a long time to form and is affected by many climate and environmental factors, such as dryness, cold, lack of oxygen, etc. or other microorganisms Interference, etc.; 3. Natural fungal grain wood is formed under natural conditions, and there is often the problem of excessive ...

Method used

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  • Phomopsis sp. fungus strain and application to fungus-line wood preparation thereof
  • Phomopsis sp. fungus strain and application to fungus-line wood preparation thereof
  • Phomopsis sp. fungus strain and application to fungus-line wood preparation thereof

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preparation example Construction

[0066] The preparation method may also include a post-processing step, which is to scrape off the mycelia on the surface of the fungal wood obtained after inoculation culture, wash and dry to a moisture content of 8-10%.

[0067] The wood-based material in step B is any one or more of logs, solid wood blocks, planks, veneers, wood flakes, sticks, and special-shaped wood products.

[0068] The tree species of the wood-based material in step B can be any tree species.

[0069] The tree species of the wood-based material described in step B are preferably poplar tomentosa, birch birch, alder wood, and pine simao.

[0070] The wood-based material described in step B can be processed into any shape as required before inoculation.

[0071] The sterilization described in step B is to sterilize the wood-based material in a high-pressure steam sterilizer at 121° C. for more than 20 minutes.

[0072] The sterilization described in step B is to place the wood-based material in a cultur...

Embodiment 1

[0080] —— Phomopsis fungi ( Phomopsis sp.) Acquisition, identification and preservation of J9GAN.

[0081] (1) Phomopsis fungus ( Phomopsis sp.) Acquisition and identification of J9GAN.

[0082] The Phomopsis fungus described in the present invention is isolated from dead branches of living trees. The samples were collected from Jindian Park, Kunming City, Yunnan Province. The dead branches of the living standing trees of broad-leaved wood with lines and patterns were collected. The length was about 3 cm and the diameter was about 1.5 cm. They were stored at 4°C for later use.

[0083] Cut the collected samples of decayed dead branches into small pieces of 0.3~0.5cm×0.3~0.5cm×0.2~0.4cm with a scalpel, soak them in 75% alcohol for 10~15s, and then soak them in 0.1% mercuric chloride for disinfection 4~6min, and then washed 3 times with sterilized distilled water. Place it in PDA medium and culture it in the dark at 28°C for 10 days until mycelium grows; then the colony i...

Embodiment 2

[0094] —— Phomopsis fungi ( Phomopsis sp.) J9GAN to prepare fungal wood.

[0095] (1) Preparation of fungal grain wood.

[0096] First, Phomopsis fungus ( Phomopsis sp.) J9GAN was inoculated on PDA liquid medium and cultured on a dark shaker at 26°C for 9 days to form mycelium with a diameter of 1-3 cm and a suspension of mycelium spores. The composition of PDA liquid medium is calculated in g / L: potato 200, glucose 18, pH 6.5-7.0.

[0097] Then, the poplar tomentosa log to be inoculated was cut into small pieces of 2cm x 2cm x 3cm, and placed in a culture bottle with a bottom diameter of 6cm and a height of 11cm. Next, pour the vermiculite into the culture bottle until it covers the wood block, add a small amount of water to moisten the wood block and vermiculite, but no excess water overflows, cover the bottle cap, put it in a high-pressure steam sterilizer, and sterilize it at 121°C. Bacteria for 60 minutes.

[0098] Clamp the wooden block and put it into the fungal s...

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Abstract

The invention discloses a phomopsis sp. fungus strain of J9GAN and an application to fungus-line wood preparation thereof, and belongs to the field of microbial technology. The strain is preserved at China general microbiological culture collection center at No. 3, Courtyard 1, Beichen West Road, Chaoyang district, Beijing, and has a preservation number of CGMCC No: 7108. The strain has excellent dip dyeing capability for wood substrates; the dip dyeing time is short and the dip dyeing depth is great; beautiful color and texture can be formed on substrate surfaces; and the physical mechanical strength is not influenced. The method for preparing fungus-line wood by using the strain comprises the steps of strain activation, amplification and propagation, and inoculation and culture. Firstly, the strain is inoculated on an activation, amplification and propagation culture medium; activation, amplification and propagation are carried out at 22-30 DEG C for 7-15 days; then a wood substrate is disinfected, and fully contacted with the amplified and propagated strains; and culture is carried out at 22-30 DEG C for 4-8 weeks. The method of the invention is reasonable in process, and easy to operate; the prepared fungus-line wood has very good decorative effect, has no influence on physical mechanical properties of the substrate, and is suitable for large-scale production, popularization and application.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Phomopsis fungus strain with short infection time and large depth, beautiful color and texture, and no influence on the physical and mechanical strength of wood-based materials and its use in the preparation of fungus-grained wood. application. Background technique [0002] The fungal discoloration of wood has long been regarded as a defect of wood, which reduces the value of wood. In fact, fungal discoloration, such as lines and stains, has natural and unique characteristics. When the decay is not serious A wonderful touch. If this kind of wood with natural lines or colors is used to make handicrafts, such as vases, earrings, pens, wood veneers, etc., the added value of wood can be increased. The fungus wood with natural patterns and colors will be popular in the market, especially in the decoration, decoration and handicraft markets. [0003] However, th...

Claims

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Application Information

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IPC IPC(8): C12N1/14B27K5/02C12R1/645
Inventor 何海珊邱坚伍建榕罗蓓伍建玲甘昌涛
Owner SOUTHWEST FORESTRY UNIVERSITY
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