Preparation method of europium-doped hydroxyapatite (HAP) fluorescent nanoparticles

Abstract

The invention relates to a preparation method of europium-doped hydroxyapatite (HAP) fluorescent nanoparticles, which comprises the following steps of 1) preparing a mixed aqueous solution of calcium chloride and europium chloride, controlling the molar ratio of Eu to Ca and Eu together at 0.1-4%, preparing an aqueous solution of disodium hydrogen phosphate, quickly pouring the aqueous solution of disodium hydrogen phosphate into the mixed aqueous solution of calcium chloride and europium chloride at room temperature, uniformly stirring and mixing, after reaction, centrifuging to obtain precipitates, washing the precipitates with deionized water, and then dispersing into the deionized water again; 2) adding a stabilizer, and performing ultrasonic dispersion treatment by use of a high-energy ultrasonic probe to obtain a stable suspension; 3) performing hydrothermal treatment on the suspension by use of a high-pressure sterilization pot to obtain a stable suspension of europium-doped HAP fluorescent nanoparticles. The method provided by the invention has the beneficial effects that effective control on the size of rare earth europium-doped HAP fluorescent nanoparticles can be realized while the crystallinity and fluorescence are improved, and the stable suspension of the europium-doped HAP fluorescent nanoparticles is obtained.

Description

technical field [0001] The invention relates to a preparation method of europium-doped hydroxyapatite fluorescent nanoparticles. Background technique [0002] The chemical composition and crystal structure of hydroxyapatite (HAP) are very similar to the inorganic salts in human bones. It has good biocompatibility and has been widely used in the treatment of bone defects. In recent years, with the development of nanotechnology, the unique properties of HAP nanomaterials have been increasingly reflected. For example, the high specific surface area of ​​HAP nanoparticles can improve the fracture toughness of ceramics, and HAP nanophase ceramics can improve bone fusion. Particles can be used as drug or gene carriers. In particular, in recent years, rare earth-doped HAP nanoparticles have also received attention as biological fluorescent probes, and are expected to become a new type of inorganic fluorescent probe material for cell labeling. [0003] Rare-earth-doped HAPs were p...

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Problems solved by technology

The former can increase its crystallinity to a certain extent, but the range of improvement is limited compared to the latter; while the latter can greatly increase its crystallinity, but the calcination treatment will cause excessive growth of grains.

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