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Concentrated Medium for Cultivating Rhodopseudomonas and Its Application

A technology for Rhodopseudomonas and culture medium, which is applied in bacteria, microorganism-based methods, microorganisms, etc., can solve the problems affecting the culture efficiency of Rhodopseudomonas, easy contamination, and a small total number of bacteria, and achieve sterilization. Convenience and thoroughness, not easy to contaminate, and the effect of reducing production costs

Active Publication Date: 2016-01-20
CHANGSHA AGREEN BIO TECH LTD CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the medium used to cultivate Rhodopseudomonas is mainly MM medium (NaCl1g / L, CaCl 2 0.5g / L, K 2 HPO 4 0.5g / L, KH 2 PO 4 0.5g / L, FeCl 3 0.02g / L, NH 4 NO 3 1g / L, MgSO 4· 7H 2 O0.03g / L, pH7.0-7.2), or add a certain amount of organic matter such as yeast extract, peptone, tryptone, etc. to MMY medium, and the prepared medium needs to be autoclaved immediately. Then use it in a very short time, otherwise, it is very easy to be polluted and cannot be used, which affects the cultivation efficiency of Rhodopseudomonas, and the total number of cultured bacteria is not much, which seriously affects its popularization and application

Method used

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  • Concentrated Medium for Cultivating Rhodopseudomonas and Its Application
  • Concentrated Medium for Cultivating Rhodopseudomonas and Its Application
  • Concentrated Medium for Cultivating Rhodopseudomonas and Its Application

Examples

Experimental program
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Effect test

Embodiment 1

[0012] Preparation of Concentrated Medium for Cultivating Rhodopseudomonas

[0013] The weight percentage component is 0.8% (NH 4 ) 2 SO 4 , 0.2%MgSO 4 , 5%NaHCO 3 , 0.4%K 2 HPO 4 , 0.15%NaCl, 0.15%NaAc, 0.05%FeSO 4· 7H 2 O, 1% yeast paste and 0.1% gelatin, the rest is water. Adjust pH=7.0 with 5mol / L NaOH. Add to 1000ml transparent plastic bag or plastic bottle, and sterilize at 0.04—0.05Mpa for 20 minutes.

Embodiment 2

[0015] Preparation of Concentrated Medium for Cultivating Rhodopseudomonas

[0016] The weight percentage component is 1% (NH 4 ) 2 SO 4 , 0.1%MgSO 4 , 4%NaHCO 3 , 0.5%K 2 HPO 4 , 0.2%NaCl, 0.2%NaAc, 0.03%FeSO 4· 7H 2 O, 1.5% yeast paste and 0.08% gelatin, the rest is water. Adjust pH=6.5 with 6mol / L NaOH. Add to 1000ml transparent plastic bag or plastic bottle, and sterilize at 0.04—0.05Mpa for 30 minutes.

Embodiment 3

[0018] The concentrated medium for cultivating Rhodopseudomonas is applied to cultivating Rhodopseudomonas palustris that efficiently degrades organophosphate and pyrethroid pesticide residues. The Rhodopseudomonas palustris is preserved in a typical culture in China National Object Collection Center, the deposit number is CCTCCNo: M2012224.

[0019] With the medium prepared in Example 1, open the bottle cap, add 9 times the volume of water, and then inoculate 1% volume of Rhodopseudomonas palustris (Rhodopseudomonaspalustris) CCTCCNo: M2012224, said Rhodopseudomonaspalustris CCTCC No: M2012224 is disclosed in the patent literature of Chinese Patent Application No. 201210394957.X, and then cultivated under natural light or incandescent lamp (25W).

[0020] Table 1 shows the number of bacteria obtained by culturing Rhodopseudomonas palustris CCTCCNo: M2012224 in the culture medium of the present invention and the MMY medium of the prior art respectively, and other culture condi...

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Abstract

The invention relates to a liquid culture medium as well as application thereof, and specifically relates to a concentrated culture medium for culturing rhodopseudomonas as well as application thereof. The concentrated culture medium comprises the following components in percentage by weight: 0.8-1% of (NH4)2SO4, 0.1-0.2% of MgSO4, 4-5% of NaHCO3, 0.4-0.5% of K2HPO4, 0.15-0.2% of NaCl, 0.15-0.2% of NaAc, 0.03-0.05% of FeSO4.7H2O, 1-1.5% of a yeast extract, 0.08-0.1% of gelatin and the balance of water. The culture medium provided by the invention is low in cost; the reproductive capacity of the cultured bacterial strains is strong and the bacterial count is great.

Description

technical field [0001] The invention relates to a liquid medium and its application, in particular to a concentrated medium for cultivating Rhodopseudomonas and its application. Background technique [0002] Microorganisms, which play an important role in ecosystems, are involved in almost every biochemical process used on Earth. With the development of technology and the deepening of research, microorganisms are widely used in various industries, including various enzyme preparations, pharmaceutical preparations, food, fuel and so on. In addition, a large number of studies have shown that in the environment, microorganisms can degrade a variety of xenobiotic substances, including many pollutants. A large number of degrading microbial resources have been isolated from various environments, and their mechanisms of biodegrading various pollutants have been gradually elucidated. [0003] However, a primary problem in the application of microorganisms is how to obtain a large ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 刘勇张松柏张德咏罗香文罗源华谭新球成飞雪程菊娥彭静朱春晖张卓
Owner CHANGSHA AGREEN BIO TECH LTD CO