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Preparation method of uridine triphosphate

A technology of uridine triphosphate and adenosine triphosphate, which is applied in the direction of fungi, fermentation, etc., can solve the problems of the influence of glycolytic enzyme activity, increased environmental pollution, complex process, etc., achieve activity retention, reduce processing steps, The effect of improving permeability

Inactive Publication Date: 2014-01-29
SHANGHAI HUIYUAN BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there will be a serious obstacle in the direct use of yeast cells, that is, nucleotide substances cannot directly enter and exit the cells. Although it can be solved by immobilization, the process of immobilizing a large number of yeast is very complicated and impractical; adding organic solvents The method is also widely used in actual production, but the added organic solvent is not recovered after synthesis, which will increase environmental pollution and have a great impact on the activity of glycolytic enzymes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Weigh 100 g of yeast cells each with a water content of about 50%, freeze them at the temperatures shown in Table 1 for 4 days, and take them out as an enzyme source to synthesize ATP. The ratio of the reaction solution is: 30mmol / L adenosine, 150mmol / L glucose, 10mmol / L magnesium sulfate, 250mmol / L pH7.5 phosphate buffer, the amount of warm yeast added is 15%, and the reaction is shaken at 30°C for 2 hours , take 1mL of the reaction solution, centrifuge to remove the yeast cells, dilute the reaction solution 10000 times with deionized water, and measure the generated ATP by HPLC. The results are shown in Table 1.

[0044] Table 1

[0045] Numbering

Embodiment 2

[0047] Weigh 100 g of yeast with each water content in Table 2, place them at -40°C for 4 days, and take them out as an enzyme source to synthesize ATP. The reaction method is as in Example 1, and the results are shown in Table 2.

[0048] Table 2

[0049] Numbering

Embodiment 3

[0051] Weigh 100 g of each yeast with a water content of about 50%, place it at -40°C for the time shown in Table 3, and take it out as an enzyme source to synthesize ATP.

[0052] The reaction method is as in Example 1, and the results are shown in Table 3.

[0053] table 3

[0054] Numbering

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Abstract

The invention provides a preparation method of uridine triphosphate, belonging to the field of biosynthesis and bioconversion. According to the method provided by the invention, high permeability yeast cells which are obtained from wet yeasts at a temperature lower than -20 DEG C for over 72 hours can efficiently convert uridylic acid into uridine triphosphate. The method of preparing the permeable yeast can maintain the integrity of yeast cells without a complex treatment process, so that the method is very applicable to industrialized production on a large scale.

Description

technical field [0001] The invention belongs to the field of biosynthesis and biotransformation, and in particular relates to a preparation method of uridine triphosphate. Background technique [0002] Uridine triphosphate (UTP) is an important biologically active substance in biological cells and participates in various biochemical reactions in the body. For example, the synthesis of polysaccharides (glycogen or starch) requires UTP to first react with glucose-1-phosphate to generate uridine diphosphate glucose (UDPG), and use this as a substrate to synthesize glycogen or starch chains; another example is ribonucleic acid ( The synthesis of RNA) requires the direct participation of UTP. [0003] In recent years, UTP has also undergone some clinical development as a drug. For example, UTP and its derivatives can be used to treat pulmonary cystic fibrosis and promote coughing of sputum in patients with lung disease (USP 5292498, Richard C.Boucher, 1994); Theodore Page uses ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/18C12P19/30C12P19/32
Inventor 欧伶张广宇邹志颜炳坤
Owner SHANGHAI HUIYUAN BIOLOGICAL SCI & TECH CO LTD
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