Mannanase and production method thereof

Abstract

The invention discloses a production method of mannanase which is suitable for an alkaline environment and has good thermal stability. The production method is characterized by comprising the steps of culturing a strain, performing shaking flask fermentation and preparing an enzyme liquid, wherein the strain is Bacillussubtilis, the laboratory number is PARK9639, the accession number is CGMCCNO: 6226, the mannanase is an alkaline enzyme, does not produce cellulase and has no activity against crystalline cellulose and carboxymethyl cellulose, and the activity of the mannanase in the pH range of 5.5-10.0 and the temperature range of 40 DEG C-60 DEG C is stable. The mannanase disclosed by the invention has high enzyme activity, the standard mannanase solution is not less than 4500u/ml, the unit production cost is low, the fermentation period is short, the degree of industrialization is high, and the technological process is simple and easy to control; and the product has no activity of the cellulase, is suitable for applications in a paper-making industry, and can reduce paper-making production cost and environmental pollution and simultaneously facilitate the improvement of product quality of paper.

Description

technical field [0001] The invention relates to the industrialized production of a biological enzyme preparation, in particular to a mannanase and a production method. Background technique [0002] Mannanase includes the following four types: (1), Mannan endo-1, 4-β-mannosidase (Mannan endo-1, 4-β-mannosidase), number EC 3.2.1.78; (2) Mannan endo-1,4-β-mannobiohydrolase (Mannan endo-1,4-β-mannobiohydrolase), number EC 3.2.1.100 [also known as exo-1,4-β-mannobiohydrolase (Exo-1, 4-β-mannobiohydrolase)]; (3) β-Mannosidase (β-Mannosidase), code number EC 3.2.1.25; (4) Mannan endo-1, 6-β-mannoside Enzyme (Mannan endo-1, 6-beta-mannosidase), number EC 3.2.1.101. [0003] As early as the beginning of the last century, there were reports on the decomposition of plant mannan. In recent years, with the continuous discovery of the use of mannanase, the development of mannanase research was promoted. In 1958, Courtios first reported the production of mannanase. The fungus of mannana...

AI Technical Summary

Problems solved by technology

However, there has been no industrial application in papermaking. The main reason is that the enzymes required for papermaking are required to be used under high pH conditions, and the enzymes are required to have good thermal stability and low price. However, most of the mannanases on the market are acid enzymes. , the effect pH is generally pH3.5~6.0, it is difficult to apply in the field of papermaking