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Method for preparing high-purity rice protein peptide through high-pressure treatment and enzymolysis

A technology of rice protein peptide and rice protein, which is applied in the field of food technology, can solve the problems of low enzymatic hydrolysis rate and heat denaturation of rice protein, achieve the goals of wide sources, reduce peroxide, improve production performance and anti-stress ability Effect

Inactive Publication Date: 2014-01-29
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high temperature (above 95°C) treatment during the above processing, the rice protein has undergone severe thermal denaturation and is almost insoluble in water.
Under normal pressure conditions, the enzymatic hydrolysis rate of rice protein is lower than 50%

Method used

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  • Method for preparing high-purity rice protein peptide through high-pressure treatment and enzymolysis
  • Method for preparing high-purity rice protein peptide through high-pressure treatment and enzymolysis
  • Method for preparing high-purity rice protein peptide through high-pressure treatment and enzymolysis

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Effect test

Embodiment 1

[0014] A method for preparing rice protein peptides by high-pressure treatment and enzymatic hydrolysis, the steps are as follows:

[0015] (1) Crushing of raw materials: Take 1Kg of rice dregs and grind them through an 80-mesh sieve;

[0016] (2) Impurity removal by amylase: Disperse the crushed rice dregs in water at a ratio of 8-10% (W / V), heat to 65°C, add high-temperature-resistant α-amylase (1500 U / kg protein), After incubating for 1.5 hours under stirring, centrifuge at 4000 rpm for 20 minutes; suspend the sediment at 12% (ratio of raw material and water) and heat to 45°C, add β-amylase, the amount of enzyme added is (500U / kg), After keeping warm for 2.5 hours under stirring conditions, centrifuge at 4000 rpm for 20 minutes; sediment washing: add hot water at 45°C at a ratio of 1:2 (ratio of raw material and water) to wash once, and the sediment is rice protein; the supernatant is recovered : The supernatant after two times of centrifugation and washing is recovered an...

Embodiment 2

[0022] A method for preparing high-purity rice protein peptides by means of high-pressure treatment, the steps are as follows:

[0023] (1) Crushing of raw materials: Take 1Kg of rice dregs and grind them through an 80-mesh sieve;

[0024] (2) Impurity removal by amylase: disperse the crushed rice dregs into water at a ratio of 10% (W / V), heat to 70°C, and add high-temperature-resistant α-amylase (1500 U / kg protein) under stirring , after incubation for 1.5 hours, centrifuge at 4000 rpm for 20 minutes; suspend the sediment at 12% (ratio of raw material and water) and heat to 45°C, add β-amylase (500 U / kg), stir After incubating for 2.5 hours under the same conditions, centrifuge at 4000 rpm for 20 minutes; sediment washing: add hot water at 45°C to wash once at a ratio of 1:2 (raw material to water ratio), the sediment is rice protein, and the supernatant is recovered: The supernatant after two times of centrifugation and washing is recovered and used for soaking the next bat...

Embodiment 3

[0030]A method for preparing high-purity rice protein peptides by high-pressure treatment and enzymolysis, the steps of which are as follows:

[0031] (1) Pulverize the rice dregs, pass through a 80-mesh sieve, disperse the rice dregs in water, the mass concentration of the rice dregs in water is 8%, soak for 1-2 hours;

[0032] (2) Heat the soaked rice dregs to 65°C, add high-temperature-resistant α-amylase, the ratio of high-temperature-resistant α-amylase to rice dregs is 1000 U / kg, keep it warm for 1.0 h under stirring, and heat at 3500 rpm Centrifuge for 20 minutes under the condition of 1 minute to obtain the rice dregs sediment for the first time; add water to the rice dregs sediment for the first time, the mass concentration of the first rice dregs sediment in water is 10%, stir and heat to 45 ℃, add β-amylase, the ratio of β-amylase to rice dregs is 500 U / kg, keep warm for 2.5 h under stirring conditions, centrifuge at 3500 rpm for 20 minutes, and obtain the second ri...

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Abstract

The invention discloses a method for preparing high-purity rice protein peptide through high-pressure treatment and enzymolysis. The method comprises the following steps: crushing and soaking rice residues, performing primary enzymolysis on the rice residues to obtain a primary rice protein, performing high-pressure treatment on the primary rice protein to obtain a secondary rice protein, performing secondary enzymolysis on the secondary rice protein to obtain a zymolyte of the rice protein, centrifuging, and performing spray drying to obtain the rice protein peptide. The adopted raw materials comprise byproducts in starch sugar production and fermentation industry and are wide in source and low in price. In the process of preparing the high-purity rice protein peptide, amylase impurity removal and high-pressure pretreatment are performed, and subsequent enzymolysis is promoted, so that the content of the finally prepared rice protein peptide is higher than 75 percent, and the content of the small peptide with molecular weight of 400-2000Da is higher than 65 percent; when the rice protein peptide is used in foods, growth and development of children are promoted, the immune function of a human body is improved, in-vivo peroxides are reduced, and senescence is delayed; when the rice protein peptide is used in animal feed, the production performance and stress resistance of animals can be improved, and the morbidity and mortality rates and sewage discharge are reduced.

Description

technical field [0001] The invention relates to a method for preparing high-purity rice protein peptides through high-pressure treatment and enzymolysis, and belongs to the field of food technology. Background technique [0002] Rice protein is recognized as a high-quality vegetable protein. It has high methionine and arginine content, low allergies, and has health functions such as anti-diabetes, anti-cholesterol, and anti-cancer. It is very suitable as a nutritional food for infants and special populations. The rice protein peptide obtained by enzymatic hydrolysis of rice protein has a variety of biological activities, such as anti-oxidation, lowering blood pressure, improving immune function, accelerating protein absorption and deposition in the body, promoting growth and development, reducing waste discharge, etc., and can be widely used in in food, cosmetic and feed products. [0003] my country's rice production accounts for one-third of the world. However, the prote...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 王章存袁道强赵学伟郑坚强李昌文
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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