Unlock instant, AI-driven research and patent intelligence for your innovation.

A visual biochip

A biochip and compound technology, applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of inability to judge the quality of chips intuitively, and achieve the effects of short detection time, high sensitivity, and simple and easy operation

Active Publication Date: 2015-09-09
NANJING XIANGZHONG BIOTECH
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, before the traditional biochip is tested, the chip product is colorless, and it is impossible to intuitively judge the quality of the chip, such as whether there are leaks, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The present invention will be further described below in conjunction with embodiment.

[0025] A visualized biochip, consisting of a carrier substrate and spotting objects on the carrier substrate, the spotting objects include antigens or antibodies or receptors of the analyte and colored conjugates or colored proteins, both in PBS buffer solution Mixing in; the colored conjugate is to couple the colored compound and the protein in the form of covalent bond.

[0026] The colored proteins include hemoglobin, fluorescent protein, hemocyanin, etc.;

[0027] The colored compound includes food coloring or colored dye colored compound;

[0028] The protein is a hydrophilic substance;

[0029] The preparation of described PBS damping fluid: prepare the PBS damping fluid of pH 7.4 with 137 mmol / L sodium chloride, 2.7 mmol / L potassium chloride, 10 mmol / L sodium dihydrogen phosphate, 2 mmol / L potassium dihydrogen phosphate .

[0030] The proteins include bovine serum albumin ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a visual biological chip. The visual biological chip is composed of a carrier substrate and point sample substances on the carrier substrate, wherein the point sample substances comprise an antigen or an antibody or a receptor of a substance to be detected, and colored conjugate or colored protein, the antigen or the antibody or the receptor of the substance to be detected and the colored conjugate or the colored protein are mixed in PBS (Phosphate Buffer Solution), and the colored conjugate is used for coupling the colored compound and the protein by a covalent bond.

Description

technical field [0001] The invention relates to a biological chip, in particular to a biological chip which is convenient for visually judging the quality of the chip and visually detecting multiple indicators in a sample to be tested. Background technique [0002] Existing biochips refer to microarrays of high-density DNA, antigens, antibodies, cells or tissues coated on immobilized carriers. Using the ability of biomacromolecules to recognize each other specifically, arrange them in an orderly manner on solid-phase carrier substrates (well plates, membranes, glass slides, polymer carriers, etc.), and simultaneously react with detection samples and labeled biomolecules Or hybridization, a large amount of useful biological information can be obtained through automatic reading equipment. At present, biochip detection methods mainly include fluorescence method, chemiluminescence method, catalytic colorimetric method and so on. Due to the high price of detection equipment suc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/535
CPCG01N33/54306G01N33/54373
Inventor 李周敏李钟卉张立柱
Owner NANJING XIANGZHONG BIOTECH