il-2 and mart-1 double gene co-expression recombinant vector and preparation method and application thereof

A MART-1, recombinant vector technology, used in gene therapy, recombinant DNA technology, and the introduction of foreign genetic material using vectors, can solve the problems of lack of immunotherapy targeting, immune regulation, and low anti-tumor ability. Good melanoma immunotherapy effect, enhanced expansion capacity and cell viability, and reduced dosage
CN103555763BActive Publication Date: 2015-11-04XINXIANG MEDICAL UNIV
1 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
XINXIANG MEDICAL UNIV
Publication Date
2015-11-04

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an IL-2 and MART-1 dual-gene co-expression recombinant vector, which is sequentially linked with an IL-2 gene, an IRES sequence and an MART-1 gene in a vector transcription direction, or is sequentially linked with an MART-1 gene, an IRES sequence and an IL-2 gene in a vector transcription direction, wherein nucleotide sequence of the IL-2 gene is represented in SEQ ID NO: 1 in a sequence list, nucleotide sequence of the MART-1 gene is represented in SEQ ID NO: 2 in the sequence list, and nucleotide sequence of the IRES sequence is represented in SEQ ID NO: 3 in the sequence list. The dual-gene co-expression recombinant vector, which links the IL-2 gene and the MART-1 gene through the IRES sequence, can simultaneously express a human melanin differentiation antigen and interleukin-2; the recombinant vector, in immunogene therapy of melanin, not only can develop an immune regulating function of a cell factor but also can generate a specific anti-tumor effect aiming at the melanin.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a recombinant vector and its preparation method and application, in particular to a double-gene co-expression recombinant vector and its preparation method and application. Background technique

[0002] Melanoma (Melanoma), also known as malignant melanoma (Malignant Melanoma, MM), is a malignant tumor derived from neural crest melanocytes. It is a malignant tumor with the highest mortality rate among surface tumors. In recent years, the incidence and corresponding mortality of melanoma are increasing rapidly, and the age of onset is getting earlier and earlier, which seriously threatens human health.

[0003] The traditional treatment of melanoma is a comprehensive treatment including surgery, chemotherapy, and immunotherapy. At present, the only effective treatment method is surgical resection of the tumor when the tumor thickness is less than 1 mm, but due to the high degree of invasion and metastasis of melanoma and resis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More