Method using quantum-dot fluorescent sensor system to determine atomization effect of protein spray
A fluorescence sensing and quantum dot technology, applied in the field of analytical chemistry, can solve the problems of poor detection sensitivity, can not meet the actual needs of the measurement of the atomization effect of protein sprays, etc., and achieve the effects of simple reaction, avoidance of deviation, and good practicability.
Inactive Publication Date: 2014-02-12
中国人民解放军63975部队
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In these two methods, HPLC is carried out by measuring the light absorption of the peptide bond at 280nm, while the BCA method is carried out by measuring the color reaction of the protein with the BCA reagent under alkaline conditions. Sensitivity is higher than that of HPLC method, but in practical applications, the detection sensitivity of these two methods is poor, and often cannot meet the actual needs of protein spray atomization effect determination
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Embodiment 1
[0023] Embodiment 1: Measuring the atomization effect of BSA protein spray with quantum dot fluorescent sensing system
[0024] Synthesis of NaHTe precursor: 10mL round bottom flask was connected with sealing water through a small needle and rubber tube, and 0.2g NaBH 4 And 0.16g Te powder joins in this sealed flask, feeds N 2 After that, inject 3.0 mL of deoxygenated distilled water under ice bath, and react for 6 hours, and the obtained clear liquid is the Te source for the next step of quantum dot synthesis;
[0025] Bifunctional TGA-CdTe quantum dot synthesis: weigh 0.23g CdCl 2After dissolving in 100mL distilled water, add NaCl, then add TGA, adjust Cd 2+ The molar ratio between TGA and TGA is 1:2.0, use 0.1M NaOH to adjust the pH of the solution to 11.2, pass N 2 30min to remove oxygen, and then quickly inject the newly synthesized sodium hydride telluride NaHTe precursor to make Cd 2+ : NaHTe molar ratio is 1:0.5, solution in N 2 Reflux at 100°C for 4 hours under p...
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The invention provides a method using a quantum-dot fluorescent sensor system to determine the atomization effect of a protein spray. The method comprises the following steps: preparing quantum dots with a good fluorescence characteristic; through coupled reaction with proteins, preparing proteins labeled by the quantum dots; using the proteins labeled by the quantum dots to replace the proteins to conduct a spray atomization experiment; determining the fluorescence intensity of a sample in a sampling pipe so as to detect the concentration of the atomized proteins, thereby determining the atomization effect of the protein spray. The system is composed of bifunctional cadmium telluride quantum dots modified by thioglycolic acid and a phosphate buffer solution with pH 7.4. The method comprises the following steps: synthetizing the quantum dots, coupling the proteins with the quantum dots to prepare a protein-quantum dot compound, purifying the protein-quantum dot compound, and determining the atomization effect of the protein spray through the protein-quantum dot compound fluorescent system. The method has the advantages of novel principle, high sensitivity, simple and easy synthetic method, simplicity in system forming and the like and is suitable for determining the atomization effect of the protein spray.
Description
technical field [0001] The invention relates to a method for measuring the atomization effect of a protein spray by using a quantum dot fluorescent sensing system, and belongs to the field of analytical chemistry. Background technique [0002] Spray is a pharmaceutical dosage form suitable for nasal administration. It is an important direction of current pharmaceutical research because of its advantages of quick onset, convenient use, little environmental impact, and simplicity. With the rapid development of biotechnology in recent decades, a large number of protein or polypeptide drugs have been put into practical use. Studies in the same period have shown that a considerable proportion of protein or polypeptide drugs can achieve therapeutic purposes through nasal administration, so a large number of Proteins or peptides are developed as sprays for nasal administration. [0003] Compared with traditional small-molecule chemically synthesized drugs, protein or polypeptide d...
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IPC IPC(8): G01N21/64
Inventor 余涛应天翼宋云扬李艳军吴方晖鹿晓晶阴忆烽刘尚义
Owner 中国人民解放军63975部队