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Application of fatty acid amide compound

A fatty acid amide and compound technology, which is applied in the application field of fatty acid amide compounds, can solve the problems of being decomposed by microorganisms, easy loss of external carbon sources, and low denitrification efficiency, so as to strengthen the rhizosphere effect, avoid water quality deterioration, and improve denitrification. The effect of nitrogen efficiency

Active Publication Date: 2015-02-11
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The substance can improve the nitrogen removal efficiency of water by promoting the activity of denitrifying microorganisms, strengthen the plant-microbe rhizosphere effect, and solve the technical problem of low nitrogen removal efficiency when dealing with low C / N farmland drainage in water pollution control in a simple way At the same time, the substance of the present invention can also improve the problem of easy loss of external carbon sources and decomposition by microorganisms, improve the denitrification efficiency of denitrifying microorganisms on farmland drainage, and save the cost of external carbon sources

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  • Application of fatty acid amide compound
  • Application of fatty acid amide compound
  • Application of fatty acid amide compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1.1 Experimental design

[0025] (1) Standard products: 4 fatty acid amide compounds, Palmitamide, Stearamide, Oleamide, Erucamide, all purchased from Sigma-Aldrich ( St.Louis, MO, USA), and dissolved in CH 2 Cl 2 middle.

[0026] (2) Microbial strains: Pseudomonas fluorescens (ACCC 01047), purchased from China Agricultural Microorganism Culture Collection Management Center.

[0027] (3) LB medium: 10g / L tryptone, 5g / L yeast extract, 10g / L NaCl, pH7.0.

[0028] (4) Denitrification medium: 0.72g / L KNO 3 , 1.0g / L KH 2 PO 4 , 0.20g / L MgSO 4 ·7H 2 O, 2.8g / LC 4 h 4 Na 2 o 4 ·6H 2 O, pH7.0.

[0029] (5) Biological activity experiment

[0030] Pseudomonas fluorescens activated in LB medium for 20 hours were collected, centrifuged for 10 minutes (5,000g, 4°C), and then resuspended in sterile denitrification medium. Add 1mL of Pseudomonas fluorescens bacteria solution and 20μL of each action substance to a 50mL Erlenmeyer flask containing 19mL of denitrification ...

Embodiment 2

[0040] Embodiment 2 Accelerator erucamide promotes denitrification efficiency of Pseudomonas fluorescens

[0041] 2.1 Experimental design

[0042] Add 5, 10, 20, 40, 60, 80mg L respectively -1 The accelerator erucamide of the present invention is added to the denitrification culture solution, and then 1 mL of Pseudomonas fluorescens bacteria solution is added, and cultivated in a shaker at 30°C and 120rpm, and the control is CH 2 Cl 2 . After culturing for 72 hours, the bacterial liquid was centrifuged according to the method in 1.1, and the total nitrogen concentration was measured in the supernatant, and the denitrification promotion rate (%) after each treatment was calculated.

[0043] 2.2 Experimental results

[0044] Accelerator of the present invention is 5-80mg L when adding concentration -1 When , the denitrification rate of Pseudomonas fluorescens can be promoted by about 5-33% ( image 3 ). Due to the addition of 60mg L -1 When the concentration is high, the d...

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Abstract

The invention relates to an application of a fatty acid amide compound. The compound provided by the invention is separated from root exudates of an aquatic plant duckweed and has a good nitrogen-removal promoting activity. With low dosage of the compound, nitrogen removal efficiency of common denitrifying bacterium pseudomonas fluorescens can be raised effectively. The compound can be used as a purifying synergist of a nitrate-polluted water body in the field of agricultural environmental protection.

Description

technical field [0001] The invention belongs to the technical research field of eutrophic water body pollution control, and specifically relates to the application of a fatty acid amide compound capable of promoting denitrification activity of denitrifying microorganisms. Background technique [0002] The first national survey of pollution sources in 2010 showed that nitrate pollution from agricultural sources has surpassed industrial sources to become the main source of total nitrogen pollution in water bodies in my country, causing eutrophication of rivers and lakes, leading to surface water pollution, and seriously threatening human health. . From a long-term perspective, excessive nitrate will lead to changes in the structure and diversity of the entire ecological community. Therefore, the purification treatment of agricultural wastewater and fertilizer water has become a new research hotspot in the field of water treatment. Using aquatic plants with high nitrogen absor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
Inventor 施卫明陆玉芳
Owner INST OF SOIL SCI CHINESE ACAD OF SCI