Method for separating and preparing anthocyanin monomer from Nitraria tangutorum Bobr

A technology of Nitraria thorn fruit and anthocyanins, which is applied to the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of volatile chemical reagents, hazards to operators, and poor separation effect, and achieve short separation time , safe operation, low cost effect

Active Publication Date: 2014-02-26
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, high-speed countercurrent chromatography (HSCCC) is mainly used for the separation of anthocyanins. The limitation is that there are fewer chromatographic peaks, poor separation effect, and long separation time; while high-speed countercurrent chromatography is used to separate anthocyanins. Due to the high polarity of anthocyanins, it is necessary to add methyl tert-butyl ether and n-butanol in the separation

Method used

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  • Method for separating and preparing anthocyanin monomer from Nitraria tangutorum Bobr
  • Method for separating and preparing anthocyanin monomer from Nitraria tangutorum Bobr
  • Method for separating and preparing anthocyanin monomer from Nitraria tangutorum Bobr

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Example 1 A method for isolating and preparing anthocyanin monomer from Nitraria thorn fruit, comprising the following steps:

[0029] (1) Raw material pretreatment: select the dried fruit of Nitraria tangutica to remove impurities, rinse with water, and wash off the surface soil to obtain the treated Nitraria fruit.

[0030] (2) Prepare a methanol solution with a volume concentration of 2% formic acid: mix 200 mL of formic acid and 9800 mL of methanol evenly.

[0031] (3) Add the methanol solution of formic acid obtained in step (2) to the fruit of Nitraria japonica, and extract at room temperature at a ratio of 1 g: 1 mL at room temperature in the dark. .

[0032] ⑷Use filter paper to remove insolubles, proteins, and polysaccharides from the extract to obtain a filtrate, which is evaporated and concentrated in a vacuum at 40°C on a rotary evaporator to a paste to obtain Nitraria anthocyanin extract, and methanol is recovered.

[0033] (5) Treatment of macropor...

Embodiment 2

[0041] Example 2 A method for isolating and preparing anthocyanin monomer from Nitraria thorn fruit, comprising the following steps:

[0042] ⑴Raw material pretreatment is the same as Example 1 .

[0043] ⑵Preparation volume concentration is 2% methanol solution of formic acid Example 1 .

[0044] (3) Add the methanol solution of formic acid obtained in step (2) to the fruit of Nitraria japonica, and extract at room temperature at a ratio of 1 g: 5 mL in the dark at room temperature.

[0045] (4) Use filter paper to remove insolubles, proteins, and polysaccharides from the extract to obtain a filtrate. The filtrate is vacuum evaporated and concentrated to a paste on a rotary evaporator at 50° C. to obtain Nitraria anthocyanin extract, and methanol is recovered.

[0046] ⑸The treatment of macroporous adsorption resin is the same as Example 1 .

[0047] (6) the macroporous adsorbent resin after the formic acid redissolution of the Nitraria anthocyanin extract with ...

Embodiment 3

[0054] Example 3 A method for isolating and preparing anthocyanin monomer from Nitraria thorn fruit, comprising the following steps:

[0055] ⑴Raw material pretreatment is the same as Example 1 .

[0056] ⑵Preparation volume concentration is 2% methanol solution of formic acid Example 1 .

[0057] (3) The formic acid methanol solution obtained in step (2) was added to the Nitraria fruit, and extracted at room temperature at a ratio of 1 g: 3 mL at room temperature and protected from light. The number of extractions was 2 times, each time for 18 h, and the extract was obtained by combining.

[0058] (4) Use filter paper to remove insolubles, proteins, and polysaccharides from the extract to obtain a filtrate, which is concentrated to a paste by vacuum evaporation on a rotary evaporator at 45° C. to obtain Nitraria anthocyanin extract, and recover methanol.

[0059] ⑸The treatment of macroporous adsorption resin is the same as Example 1 .

[0060] 6. The macroporou...

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Abstract

The invention relates to a method for separating and preparing anthocyanin monomer from Nitraria tangutorum Bobr, which comprises the following steps: (1) raw material pretreatment: roguing dry Tangut Nitraria tangutorum Bobr, and washing with water to obtain the treated Nitraria tangutorum Bobr; (2) preparing a formic acid methanol solution of which the volume concentration is 2%; (3) adding the formic acid methanol solution into the Nitraria tangutorum Bobr, and carrying out normal temperature leaching to obtain an extract; (4) filtering the extract, and concentrating by evaporation to obtain a Nitraria tangutorum Bobr anthocyanin extractum; (5) treating with a macroporous adsorbent resin; (6) redissolving the Nitraria tangutorum Bobr anthocyanin extractum, passing through the treated macroporous adsorbent resin, and collecting the effluent liquid; (7) concentrating the effluent liquid under reduced pressure to obtain an anthocyanin crude product; (8) injecting the anthocyanin crude product into a high-performance semipreparative chromatographic column to perform separation, thereby obtaining the anthocyanin monomer with the purity of at least 90%; and finally, concentrating the anthocyanin monomer reduced pressure, and drying to constant weight, thereby obtaining the anthocyanin monomer finished product. The method has the advantages of favorable separating effect, short time consumption and low cost.

Description

technical field [0001] The invention relates to a method for separating and preparing anthocyanin monomers, in particular to a method for separating and preparing anthocyanin monomers from Nitraria thorn fruit. Background technique [0002] Tangut Nitraria ( Nitraria Tangutorum Bobr) is a plant of the genus Nitraria (Zygophyllaceae) of the family Zygophyllaceae. It is a medicinal and edible plant that mainly grows in desert or semi-desert areas. The fruit of Nitraria thorn contains anthocyanins, total polyphenols, polysaccharides, amino acids, vitamins, flavonoids, alkaloids, mineral elements and other components, which have significant functions of lowering blood sugar, blood fat, regulating immunity, anti-fatigue, cold resistance, anti-oxidation, Biological activities such as anti-aging, liver protection, anti-tumor and anti-cancer. After the Nitraria fruit matures, it presents different colors, and its fruit color has a variety of colors such as yellow, light red, brig...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08C09B61/00
Inventor 索有瑞胡娜丁晨旭张秋龙
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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