Method for separating and preparing anthocyanin monomer from Nitraria tangutorum Bobr

Abstract

The invention relates to a method for separating and preparing anthocyanin monomer from Nitraria tangutorum Bobr, which comprises the following steps: (1) raw material pretreatment: roguing dry Tangut Nitraria tangutorum Bobr, and washing with water to obtain the treated Nitraria tangutorum Bobr; (2) preparing a formic acid methanol solution of which the volume concentration is 2%; (3) adding the formic acid methanol solution into the Nitraria tangutorum Bobr, and carrying out normal temperature leaching to obtain an extract; (4) filtering the extract, and concentrating by evaporation to obtain a Nitraria tangutorum Bobr anthocyanin extractum; (5) treating with a macroporous adsorbent resin; (6) redissolving the Nitraria tangutorum Bobr anthocyanin extractum, passing through the treated macroporous adsorbent resin, and collecting the effluent liquid; (7) concentrating the effluent liquid under reduced pressure to obtain an anthocyanin crude product; (8) injecting the anthocyanin crude product into a high-performance semipreparative chromatographic column to perform separation, thereby obtaining the anthocyanin monomer with the purity of at least 90%; and finally, concentrating the anthocyanin monomer reduced pressure, and drying to constant weight, thereby obtaining the anthocyanin monomer finished product. The method has the advantages of favorable separating effect, short time consumption and low cost.

Description

technical field [0001] The invention relates to a method for separating and preparing anthocyanin monomers, in particular to a method for separating and preparing anthocyanin monomers from Nitraria thorn fruit. Background technique [0002] Tangut Nitraria ( Nitraria Tangutorum Bobr) is a plant of the genus Nitraria (Zygophyllaceae) of the family Zygophyllaceae. It is a medicinal and edible plant that mainly grows in desert or semi-desert areas. The fruit of Nitraria thorn contains anthocyanins, total polyphenols, polysaccharides, amino acids, vitamins, flavonoids, alkaloids, mineral elements and other components, which have significant functions of lowering blood sugar, blood fat, regulating immunity, anti-fatigue, cold resistance, anti-oxidation, Biological activities such as anti-aging, liver protection, anti-tumor and anti-cancer. After the Nitraria fruit matures, it presents different colors, and its fruit color has a variety of colors such as yellow, light red, brig...

AI Technical Summary

Problems solved by technology

[0004] At present, high-speed countercurrent chromatography (HSCCC) is mainly used for the separation of anthocyanins. The limitation is that there are fewer chromatographic peaks, poor separation effect, and long separation time; while high-speed countercurrent chromatography is used to separate anthocyanins. Due to the high polarity of anthocyanins, it is necessary to add methyl tert-butyl ether and n-butanol in the separation system. These two highly polar reagents are difficult to remove in the subsequent concentration process, and even if they can be removed, high temperatures are required. will cause loss of anthocyanins

Furthermore, high-speed countercurrent chromatography is an open separation system, and the chemical reagents, especially methyl tert-butyl ether, are easy to volatilize, causing harm to operators and easy to pollute the environment.

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