Preparation method of benzothiostrobin hapten, artificial antigen and specific antibody and application thereof
A technology of benthiazolin and antibody heavy chain, applied in chemical instruments and methods, specific peptides, animal/human proteins, etc., to achieve the effects of easy popularization, low analysis cost, and large analysis capacity
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[0022] 1 Synthesis of artificial hapten
[0023] Alkaline hydrolysis of the carboxylate on the benzene ring of penthiostrobin to synthesize a hapten with a three-atom linking arm, which not only retains the characteristic group of penthiostrobin to the greatest extent, but also forms a carboxyl terminal group, which can Better for coupling to proteins. The product was purified by mass spectrometry (ESI) and H NMR spectroscopy ( 1 H-NMR) identification. The molecular structural formula of the artificial hapten of benzathystrobin is:
[0024]
[0025] 1.1 Synthesis of artificial haptens
[0026] Weigh 8.02g (0.02mol) of penthiostrobin technical substance and 30mL of tetrahydrofuran into a 100mL conical flask, then add 10.08mL of 5% lithium hydroxide solution (penthiostrobin:lithium hydroxide=1:1.05), Stir the reaction at room temperature for 4 hours, add 20 mL of water, extract three times with ethyl acetate, 40 mL each time, discard the organic phase, adjust the pH value...
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