Giant salamander oligosaccharide peptide health-care product
A technology of giant salamander oligosaccharide peptides and health products, applied in polysaccharide/gum-containing food ingredients, food preparation, food extraction, etc., to achieve unique formula, simple ingredients, and the effect of increasing white blood cells
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Embodiment 1
[0024] The preparation of embodiment 1 raw material
[0025] Preparation of giant salamander oligosaccharide peptides: Homogenize giant salamander mucus at 0°C, add 3 times the volume of 0.1M phosphate buffer with pH 7-8 to the homogenate, and a compound enzyme with a mass ratio of 0.1% to the homogenate , enzymatic hydrolysis for 48 hours, centrifuged to take the supernatant, separated by an ultrafiltration membrane separator with a molecular weight cut-off of 4000Da or less, took the liquid that passed through the ultrafiltration membrane, passed through a SephadexLH-20 molecular sieve chromatography column, and collected OD 280nm Absorption peak, the collected liquid is passed through the macroporous adsorption resin and activated carbon, and then freeze-dried to obtain the giant salamander oligosaccharide peptide. The ratio is 3:3:2:2 ratio mixing;
[0026] Preparation of Cordyceps polysaccharide: take Cordyceps mycelium as raw material, add 70-95% ethanol to reflux extra...
Embodiment 2
[0028] The preparation of embodiment 2 raw materials
[0029]Preparation of giant salamander oligosaccharide peptides: Homogenize giant salamander mucus at 10°C, add an equal volume of 0.01M phosphate buffer with pH 7-8, and a compound enzyme with a mass ratio of 1% to the homogenate, Enzyme hydrolyze for 12 hours, centrifuge to take the supernatant, separate with an ultrafiltration membrane separator with a molecular weight cut-off of 4000Da or less, take the liquid that has passed through the ultrafiltration membrane, pass through a SephadexLH-20 molecular sieve chromatography column, and collect OD 280nm Absorption peak, the collected liquid is passed through the macroporous adsorption resin and activated carbon, and then freeze-dried to obtain the giant salamander oligosaccharide peptide. The ratio is 3:3:2:2 ratio mixing;
[0030] Preparation of Cordyceps polysaccharide: take Cordyceps mycelium as raw material, add 70-95% ethanol to reflux extraction, each time for 3 hou...
Embodiment 3
[0032] The preparation of embodiment 3 raw materials
[0033] Preparation of giant salamander oligosaccharide peptides: Homogenize giant salamander mucus at 5°C, add 2 times the volume of 0.05M phosphate buffer with pH 7-8 and a compound enzyme with a mass ratio of 0.6% to the homogenate, Enzyme hydrolysis for 24 hours, centrifuge to take supernatant, use ultrafiltration membrane separator with molecular weight cut-off below 4000Da to separate, take the liquid that has passed through the ultrafiltration membrane, pass through SephadexLH-20 molecular sieve chromatography column, and collect OD 280nm Absorption peak, the collected liquid is passed through the macroporous adsorption resin and activated carbon, and then freeze-dried to obtain the giant salamander oligosaccharide peptide. The ratio is 3:3:2:2 ratio mixing;
[0034] Preparation of Cordyceps polysaccharide: take Cordyceps mycelium as raw material, add 70-95% ethanol to reflux to extract and degrease, 4 hours each ti...
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