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Airlift type magnetic anion bioreactor

A bioreactor and negative ion technology, applied in the field of biochemical cultivation in life science laboratories, can solve the problems of liquid culture efficiency reduction, unfavorable, bacterial species or cell decline, etc., to improve cell growth vigor and growth, increase production time and Yield, effect of promoting metabolism

Inactive Publication Date: 2015-03-25
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This will inevitably cause the efficiency of liquid culture to be greatly reduced due to air pollution. For example, excessive sulfur dioxide in the air will lead to rapid decline or poisoning of strains or cells, and a sharp reduction in the output of target products.
Moreover, the oxygen ratio in the air is fixed, so the traditional airlift liquid culture tank is not conducive to the liquid culture research and production needs of adjusting the oxygen ratio

Method used

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  • Airlift type magnetic anion bioreactor
  • Airlift type magnetic anion bioreactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Such as figure 1 As shown, an airlift type magnetic negative ion bioreactor includes an electrolytic oxygen tank 1, a magnetic field-high voltage generator 2 arranged on the oxygen path on the electrolytic oxygen tank 1, an electrolytic positive electrode 3 and an electrolytic negative electrode 4 , oxygen electrode 5 and power controller 6, liquid culture tank 11 and its bottom valve 10, air circulation channel 12 and its internal temperature controller 7, feed pipe 8 and air pump 9, pH meter and thermometer connected with the controller 13. The sampling port and its valve 14, the tank top cover 15, and the air filter 16 on the air circulation channel 12.

[0022] The electrolysis positive electrode 3 of the electrolysis oxygen water tank 1 releases oxygen into the magnetic field-high voltage generator 2 through the electrolysis of water, and the magnetic field located on the air circulation channel 12-the high voltage electrodes of the high voltage generator 2 go deep...

Embodiment 2

[0026] Example 2: Application example in aerobic cell culture.

[0027] Take the fermentation of Cordyceps militaris mycelium as an example:

[0028] Step 1. First, set the temperature controller according to the suitable temperature for the growth of the Cordyceps militaris strain. The temperature range in the liquid culture tank 11 is controlled at 15-16 degrees Celsius. The aseptic operation injects the sterile medium into the tank. The components of the sterile culture medium include deionized water, yeast extract, glucose, potassium dihydrogen phosphate and zinc sulfate, and the mass ratio is: 1500(g):5(g): 50(g):0.3(g):0.3(g), inoculated with Cordyceps militatirs under aseptic conditions.

[0029] Step 2, turn on the air pump 9, the gas in the air circulation channel 12 starts to circulate, stir the fermentation liquid in the liquid culture tank 11, and set the dissolved oxygen rate range of 16-18mg / L and pH value range for the power controller 6 At 6.0-7.5.

[0030] ...

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Abstract

The invention discloses an airlift type magnetic anion bioreactor. The airlift type magnetic anion bioreactor comprises a liquid cultivation tank, wherein a tank top cover is arranged at the top of the liquid cultivation tank; the bottom of the liquid cultivation tank is communicated with the top of the liquid cultivation tank through an air-circulating passage; an air pump for circulating air and a temperature controller for heating the temperature in the air-circulating passage are arranged in the air-circulating passage; the air-circulating passage is further sleeved with a magnetic field-high pressure generator. The airlift type magnetic anion bioreactor further comprises an electrolytic oxygen water tank, wherein the output pipeline of the electrolytic positive electrode of the electrolytic oxygen water tank is communicated with the air-circulating passage. The airlift type magnetic anion bioreactor also comprises an oxygen electrode arranged in the liquid cultivation tank and used for detecting the oxygen dissolution rate, as well as a pH meter and a thermometer which are used for detecting the pH value and the temperature respectively. According to the invention, oxygen required by cells, tissues or organisms in a culture solution is supplied by electrolytic oxygen; the anion generation method is adopted to adjust the pH value and the oxidation-reduction potential of the culture solution and neutralize oxidants or free radicals generated in the cell growing process.

Description

technical field [0001] The invention belongs to the technical field of biochemical cultivation in a life science laboratory, and more specifically relates to an airlift type magnetic negative ion bioreactor, which is suitable for the liquid culture research of autotrophic cells, heterotrophic cells, and animal and plant cells or tissues. Background technique [0002] Biochemical engineering is one of the high-tech directions in the field of life sciences. It is one of the development trends of life sciences to produce medicines, food and even tissues or organs needed by human beings by means of biochemical engineering. In the basic research and industrial application of biochemical reactions, cell culture is the most commonly used method to study the synthesis of target products by wild-type cells and engineered cells. Airlift liquid culture is widely used due to its gentle agitation and easy control. However, the current air-lift culture method of aerobic microorganisms a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/42C12M1/38C12M1/36C12M1/34C12M1/04
CPCC12M29/08C12M29/26C12M37/02C12M41/12C12M41/26C12M41/32
Inventor 董静洲朱蕾雷灿王瑛艾训儒郑小江
Owner HUBEI UNIV FOR NATITIES
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