Method for determining betaine content in wolfberry
A method of determination, the technology of Lycium barbarum, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor reproducibility, high condition requirements, and many interference factors, and achieve high accuracy, good repeatability, and simple operation Effect
Inactive Publication Date: 2014-03-12
广东汇群中药饮片股份有限公司
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Problems solved by technology
However, there are many interference factors in this method, and the temperature and humidity have a great influence on the color rendering, and the reproducibility is poor.
As a modern separation and analysis technique, high performance liquid chromatography has been gradually applied to the separation, analysis and content determination of the chemical components of traditional Chinese medicines. It is also widely used in the determination of plant betaine content. However, because there is no conjugated system in the structure of betaine , can only be detected with a refractometer or measured at a low ultraviolet absorption wavelength (190-220nm), the conditions are relatively high, and the sensitivity or selectivity are not ideal
Therefore, the commonly used HPLC method is to measure the betaine extract after pre-column derivatization, but this method destroys the original components of the betaine extract
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[0038] The content determination method of betaine in fruit of wolfberry comprises the following steps:
[0039] (1) Prepare the test solution:
[0040] A) Cut wolfberry fruit into pieces, take 2g of wolfberry fruit, put it in a reflux bottle, add 50ml of methanol, seal the reflux bottle with a bottle cap, weigh the whole reflux bottle, heat and reflux for 1 hour to obtain a wolfberry solution, and let it cool , weigh the weight of the overall reflux bottle again, make up the lost weight with methanol, shake well, filter, and measure 10ml of filtrate for subsequent use;
[0041] B) Take 3g of basic alumina with a particle size of 100-200 mesh, put it into a chromatographic column with an inner diameter of 10-15mm, pre-wash the basic alumina with 20ml of ethanol, and add the subsequent filtrate prepared in step A to In the basic alumina column, elute with 100ml of ethanol to remove the active ingredients in the wolfberry solution, collect the eluate, evaporate to dryness, add ...
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Abstract
The invention discloses a method for determining betaine content in wolfberry. Conventional measuring methods are easily influenced by external factors, are bad in repeatability and cannot help to completely preserve effective components of betaine. According to the method, DAD-HPLC method is employed for determining the content of betaine in wolfberry, and by determining the linear relation, the precision and the stability of the peak area response value, the sample recovery rate and the like of betaine, the determining method helps to master the content of betaine in wolfberry. The determining method is simple in operation, high in accuracy and good in repeatability, and is worth popularization and application for determining on betaine content in wolfberry; and the method provided by the invention is widely applied to betaine content determining in various traditional Chinese medicinal materials especially in wolfberry.
Description
technical field [0001] The invention relates to a content determination method, in particular to a content determination method of betaine in wolfberry. Background technique [0002] Lycium barbarum is the dry and mature fruit of Lycium barbarum of the Solanaceae plant. It has the functions of nourishing liver and kidney, benefiting energy and improving eyesight. Betaine is one of the main alkaloids in the fruits, leaves and stalks of Lycium barbarum. It acts as a methyl donor in the body and is the main active substance in Lycium barbarum for lipid metabolism or anti-fatty liver. "Chinese Pharmacopoeia" 2010 edition adopts thin-layer chromatography to determine the content of betaine in Lycium barbarum. This method uses methanol and n-butanol to extract samples, and develops them on silica gel thin-layer plates with different proportions of chloroform-methanol-water or other developing agents. After color development with modified bismuth iodide potassium reagent, scanning...
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IPC IPC(8): G01N30/02
Inventor 陈潮升
Owner 广东汇群中药饮片股份有限公司