Method for detecting deer-derived ingredients in glue type traditional Chinese medicines and products thereof

A technology of glue-like traditional Chinese medicine and source components, which is applied in measurement devices, material separation, analysis of materials, etc., can solve the problem of no traceability identification and other problems, and achieve the effect of less damage and high content

Active Publication Date: 2014-03-12
SHAN DONG DONG E E JIAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no method for traceability and iden...

Method used

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  • Method for detecting deer-derived ingredients in glue type traditional Chinese medicines and products thereof
  • Method for detecting deer-derived ingredients in glue type traditional Chinese medicines and products thereof
  • Method for detecting deer-derived ingredients in glue type traditional Chinese medicines and products thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0029] (1) Preparation of gel sample enzymatic hydrolysis solution

[0030] Take 1.0g of the gel sample to be tested in a 500mL measuring bottle, add a small amount of 1%NH 4 HCO 3 solution, sonicate to dissolve the sample completely, and use 1% NH 4 HCO 3 Dilute the solution (pH 8.0) to the mark, shake well, filter through a microporous membrane, accurately measure 1 mL of the subsequent filtrate plus 100 μL of 2 mg / mL trypsin solution, and enzymatically hydrolyze at a constant temperature of 37 °C for 6 h.

[0031] (2) Preparation of enzymatic hydrolysis solution of synthetic polypeptide control sample

[0032] Weigh a certain amount of synthetic peptide, add 1% NH 4 HCO 3 Solution (pH8.0), shake well, prepare to a concentration of about 0.3μg / mL, filter through a microporous membrane, and set aside.

[0033] 3 Discovery of peptides

[0034] (1) Discovery of ions

[0035] Take 5 μL of the enzymatic hydrolysis solution and put it into the liquid mass spectrometer for ...

Embodiment 1

[0045] 1 Materials and Reagents

[0046] Materials: Pure glue includes donkey-hide gelatin, horsehide glue, yellow gelatin, pigskin glue, tortoise shell glue, and deer antler glue, which are obtained by decocting donkey hide, horse hide, cowhide, pig skin, tortoise shell, and antler respectively.

[0047] Mixed gum samples (donkey-hide gelatin with 5% antler gum, donkey-hide gelatin with 10% antler gum, donkey-hide gelatin with 20% antler gum, and donkey-hide gelatin with 40% antler gum) were made by precisely mixing the above pure gums. (percentage is mass percent)

[0048] Reagents: ammonium bicarbonate (analytical pure), trypsin (sequentially pure, purchased from China Institute for Food and Drug Control), synthetic polypeptide Gly-Glu-Ala-Gly-Pro-Gln-Gly-Pro-Arg.

[0049] 2 detection method

[0050] (1) Preparation of synthetic peptide control sample enzymatic hydrolysis solution

[0051] Get 1.0g pure donkey-hide gelatin sample in 500mL measuring bottle, add a small am...

Embodiment 2

[0058] 1 Materials and Reagents

[0059] Materials: Mixed glue samples are made by adding 5% antler glue to the pure glue sample in Example 1, including donkey-hide gelatin containing 5% antler glue, horsehide glue with 5% antler glue, and yellow gelatin containing 5% antler glue , Pig skin glue with 5% antler glue, tortoise shell glue with 5% antler glue. (percentage is mass fraction)

[0060] Reagents: ammonium bicarbonate (analytical pure), trypsin (sequentially pure, purchased from China Institute for Food and Drug Control), synthetic polypeptide Gly-Glu-Ala-Gly-Pro-Gln-Gly-Pro-Arg.

[0061] 2 detection method

[0062] (1) Preparation of synthetic peptide control sample enzymatic hydrolysis solution

[0063] Get 1.0g sample of pure donkey-hide gelatin to be tested in a 500mL measuring bottle, add a small amount of 1%NH 4 HCO 3 solution, sonicate to dissolve the sample completely, and use 1% NH 4 HCO 3 Dilute the solution (pH 8.0) to the mark, shake well, filter with...

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Abstract

The invention provides a method for detecting deer-derived ingredients in glue type traditional Chinese medicines and products thereof. The method provided by the invention comprises the step of detecting by using the difference between the specific nucleotide sequence or amino acid sequence of a genome of a red deer and the specific nucleotide sequence or amino acid sequence of a genome of a spotted deer, wherein the specific amino acid sequence is shown in SEQ ID No. 1. By adopting the method provided by the invention, whether the glue type traditional Chinese medicines contain the deer-derived ingredients or not can be detected rapidly, so as to distinguish the authenticity; although polypeptides of collagen in the glue type traditional Chinese medicines are subjected to certain hydrolysis and destruction, the deer-derived ingredients in the glue type traditional Chinese medicines and products thereof can be identified on the basis that differential polypeptides in a main ingredient, namely collagen, of the glue type traditional Chinese medicines are high in content and little in damaged extent.

Description

technical field [0001] The invention relates to a detection method of deer-derived components, in particular to a detection method of deer-derived components in gum traditional Chinese medicine and its products, and belongs to the field of detection of traditional Chinese medicines. Background technique [0002] Antlers, that is, the ossified horns of red deer (Cervus elaphus Linnaeus) or sika deer (Cervus nippon Temminck) or the horn bases that fall off in the next spring after sawing the velvet, belong to traditional Chinese medicine. According to the latest version of the Chinese Pharmacopoeia, taking deer antlers can warm kidney yang, strengthen muscles and bones, promote blood circulation and reduce swelling. Antler glue made from antlers occupies an important position in the entire Chinese medicine market. [0003] According to the 2010 edition of the Chinese Pharmacopoeia, deer horn glue is a solid glue made of deer horn as raw material, decocted and concentrated. It...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 周祥山秦玉峰尤金花田守生嵇传良郭尚伟段小波史兆松
Owner SHAN DONG DONG E E JIAO
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