Method for measuring contents of six amino acids in ixeris sonchifolia injection by using pre-column derivatization method

A pre-column derivatization and bitter dish technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as large content, and achieve the effects of good reproducibility, high accuracy, and easy operation

Inactive Publication Date: 2014-03-12
TONGHUA HUAXIA PHARMA
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Problems solved by technology

Traditional Chinese medicine injections are varieties with high safety risks, and the requirements of relevant standards are relatively high. The current research on the quality control of Kudiezi injection mainly focuses on the content determination of flavonoids, sugars and organic acids. The content of Dizi injection is relatively large and has a clear medicinal effect

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  • Method for measuring contents of six amino acids in ixeris sonchifolia injection by using pre-column derivatization method
  • Method for measuring contents of six amino acids in ixeris sonchifolia injection by using pre-column derivatization method
  • Method for measuring contents of six amino acids in ixeris sonchifolia injection by using pre-column derivatization method

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Embodiment Construction

[0025] Pre-column derivatization method for determination of 6 kinds of amino acids in Kudiezi injection:

[0026] 1. Instruments and Materials

[0027] Waters-e2695 high performance liquid chromatography system (including quaternary pump, online vacuum degasser, automatic sampler, column thermostat, 2998 photodiode array detector; Empower 2 chromatography workstation; American Waters company); BT125D analytical balance [Sartorius Scientific Instrument (Beijing) Co., Ltd.]; THZ-82 water bath constant temperature oscillator (Jintan Ronghua Instrument Manufacturing Co., Ltd.); KH 2200B ultrasonic cleaner (Kunshan Hechuang Ultrasonic Instrument Co., Ltd.)

[0028] Proline, valine, alanine, phenylalanine, leucine, isoleucine reference substances (National Institute for the Control of Pharmaceutical and Biological Products, batch number: 140624-200805); the purity of all reference substances was determined by HPLC peak area Normalization method calculation is all greater than...

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Abstract

The invention relates to a method for measuring contents of six amino acids in an ixeris sonchifolia injection by using a pre-column derivatization method, belonging to the field of traditional Chinese medicine ingredient analysis. The method comprises the following steps: (1) preparing a mixed reference substance solution containing 217.0mg.L<-1> of proline, 37.40mg.L<-1> of alanine, 44.40mg.L<-1> of valine, 39.80mg.L<-1> of isoleucine, 43.20mg.L<-1> of leucine and 42.20mg.L<-1> of phenylalanine; (2) preparing a solution of test articles; and (3) carrying out gradient elution according to the following chromatographic conditions: a DiamonsilC18(2) chromatographic column (200*4.6mm, 5mu m), 25 DEG C of column temperature, 350nm of detection wavelength, 1.0mL/min of flow velocity, 10mu L of sample amount, a mobile phase A, namely water, a mobile phase B, namely 30mmol/L NaAc (containing 1% of N,N-dimethylfomamide), 6.40 of pH value and a mobile phase C, namely acetonitrile. The method has the advantages of simplicity, convenience, easiness in operation, high accuracy, good repeatability and the like and lays the foundation for research of the quality control method of the ixeris sonchifolia injection.

Description

[0001] technical field [0002] The invention relates to a pre-column derivatization method for determining the content of six amino acids in Kudiezi injection. Belongs to the field of traditional Chinese medicine component analysis. Background technique [0003] In the prior art, Kudiezi has another name called Gypsophila gypsophila, and it is mainly distributed in Northeast my country and Inner Mongolia. Grass, cold in nature, bitter and pungent in taste, has the effects of clearing heat and detoxifying, draining pus and relieving pain. Kudiezi (Diemailing) Injection is an intravenous injection made from Kudiezi extracted and refined, which has the functions of promoting blood circulation, removing blood stasis and improving microcirculation [2] . The injection has been on the market for many years, and its curative effect is definite. Kudiezi Injection (former name: Diemailing Injection) is included in the national drug standard product (Z20025450), produced by Tonghua H...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 刘睿李遇伯张艳军孙璐
Owner TONGHUA HUAXIA PHARMA
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