Preparation method of nano sulfonated graphene and application of nano sulfonated graphene as gene transfer material
A technology of sulfonated graphene and graphene, which is applied in the direction of graphene, chemical instruments and methods, nano-carbon, etc., can solve the problems of low transfection efficiency, high production cost, and limited penetration of gene guns, and achieves the preparation method Simple, low production cost, reproducible results
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Embodiment 1
[0045] A kind of preparation method of nanometer sulfonated graphene, it comprises the following steps:
[0046] Step 1, prepare graphene oxide by Hummers method: prepare graphene oxide by Hummers method with graphite powder, potassium permanganate, sodium nitrate and concentrated sulfuric acid;
[0047] Step 2, the preparation of reduced graphene oxide:
[0048] (1) Disperse graphene oxide in deionized water, and then perform ultrasonic treatment for a certain period of time to obtain a graphene oxide dispersion system; wherein, the mass ratio of deionized water to graphene oxide is 1000:1;
[0049](2) adding sodium carbonate solution to the dispersion system of graphene oxide, so that the pH value of the dispersion system of graphene oxide is 9;
[0050] (3) Add sodium borohydride solution to the above-mentioned graphene oxide dispersion system with a pH value of 9, and then stir at 80°C for 1 hour to obtain reduced graphene oxide, and then wash the reduced graphene oxide w...
Embodiment 2
[0060] A kind of preparation method of nanometer sulfonated graphene, it comprises the following steps:
[0061] Step 1, prepare graphene oxide by Hummers method: prepare graphene oxide by Hummers method with graphite powder, potassium permanganate, sodium nitrate and concentrated sulfuric acid;
[0062] Step 2, the preparation of reduced graphene oxide:
[0063] (1) Disperse graphene oxide in deionized water, and then perform ultrasonic treatment for a certain period of time to obtain a graphene oxide dispersion system; wherein, the mass ratio of deionized water to graphene oxide is 980:1;
[0064] (2) adding sodium carbonate solution to the dispersion system of graphene oxide, so that the pH value of the dispersion system of graphene oxide is 10;
[0065] (3) Add sodium borohydride solution to the above-mentioned graphene oxide dispersion system with a pH value of 10, and then stir at 75°C for 1.2 hours to obtain reduced graphene oxide, and then wash the reduced graphene ox...
Embodiment 3
[0075] A kind of preparation method of nanometer sulfonated graphene, it comprises the following steps:
[0076] Step 1, prepare graphene oxide by Hummers method: prepare graphene oxide by Hummers method with graphite powder, potassium permanganate, sodium nitrate and concentrated sulfuric acid;
[0077] Step 2, the preparation of reduced graphene oxide:
[0078] (1) Disperse graphene oxide in deionized water, and then perform ultrasonic treatment for a certain period of time to obtain a graphene oxide dispersion system; wherein, the mass ratio of deionized water to graphene oxide is 1020:1;
[0079] (2) adding sodium carbonate solution to the dispersion system of graphene oxide, so that the pH value of the dispersion system of graphene oxide is 9.5;
[0080] (3) Add sodium borohydride solution to the above-mentioned graphene oxide dispersion system with a pH value of 9.5, and then stir at 85°C for 0.8 hours to obtain reduced graphene oxide, and then wash the reduced graphene...
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