Achromobacter marplatensis and application thereof
An endophytic bacteria, sugar beet technology, applied in the application, bacteria, biocide and other directions, can solve the problem that the growth promotion effect of beet endophytes has not been reported, and achieve good technical effects, increase sugar content, and promote growth.
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Embodiment 1
[0032] Example 1: sugar beet endophytic bacteria Achromobacter marplatensis Isolation, screening and identification of TNSC2011 CGMCC No. 8192
[0033] (1) Isolation and screening of strains
[0034] Several strains of IAA-producing bacteria were isolated from healthy beet by gradient dilution method, and a strain numbered TNSC2011 was screened out, which can effectively promote crop growth and convert insoluble phosphate into bioavailable soluble phosphoric acid Salt, improves photosynthesis. It has outstanding growth-promoting effect through application in sugar beet cultivation.
[0035] The colonies of this strain are small and smooth, transparent, white, with moist surface, neat edges, and fast growth; through Gram staining and microscopic observation, it is found that the strain is Gram-negative bacteria. Microbiologically identified as Achromobacter marplatensis Bacteria, rod-shaped cells, 0.8-1.0um×1.5-2um, Gram-negative (-), no spores, medium and smooth colo...
Embodiment 2
[0047] Embodiment two: sugar beet endophytic bacteria Achromobacter marplatensi s Growth factors of TNSC2011 CGMCC No. 8192
[0048] But should beet endophytic bacteria provided according to the present invention Achromobacter marplatensis TNSC2011 CGMCC No. 8192 was characterized to determine its specific growth factors, and the strain TNSC2011 was inoculated and cultivated. The results are shown in Table 2.
[0049] Table 2: Effects of temperature, pH, salt, and antibiotics on the growth of strain TNSC2011
[0050] temperature (°C) 4 15 25 30 32 35 growing situation — + + +++ ++++ +++ temperature (°C) 38 45 50 growing situation ++ - — pH 1 2 3 4 5 6 growing situation — — — — + ++ pH 7 8 9 10 growing situation +++ + — — NaCl concentration 0.5% 1% 2% 3% 4% 5% growing situation + ++ +++ +++ +++ + NaCl concentration 6% 7% 8% 9% ...
Embodiment 3
[0057] Embodiment three: sugar beet endophytic bacteria Achromobacter marplatensis Research on the fermentation process of TNSC2011 CGMCC No. 8192
[0058] The purpose of obtaining preparations by fermentation is achieved through the optimization of carbon, nitrogen sources, pH value, etc.
[0059] (1) Determination of medium pH
[0060] According to the pH value of 6.0, 6.5, 7.0, 7.5, 8.0, ferment at 120 r / min, temperature 35 ℃, the optimum growth is obtained.
[0061] (2) Optimization of carbon and nitrogen sources
[0062] Seed liquid medium: 2.0% corn flour, 0.5% yeast extract, 7.5% corn steep liquor, KH 2 PO 4 0.1%, K 2 HPO 4 0.1%, MgSO 4 ·7H 2 00.05%, continue to optimize on the basis of seed culture medium;
[0063] A. Carbon source optimization:
[0064] The carbon sources are glucose, sucrose, corn flour, soluble starch, and other components are KH 2 PO 4 0.1%, K 2 HPO 4 0.1%, MgSO 4 ·7H 2 O0.05% PH7.0;
[0065] B. Nitrogen source optimizatio...
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