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Method for improving soy isoflavone conversion rate of composite probiotics fermented soybean meal feed

A technology of isoflavone conversion rate and compound probiotics, applied in animal feed, animal feed, application, etc., can solve the problems of harmful body health, harsh reaction conditions, low conversion efficiency, etc., achieve high recovery rate, lower pH value, The effect of improving the cleaning ability

Inactive Publication Date: 2014-03-26
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such as invention patents CN1315322, CN1315323 and CN1448394, U.S. Patent (US5554519A), Japanese Patent (JP11-89589, JP8-214787) all disclose the method for producing soybean isoflavone aglycon by enzymatically hydrolyzing the sugar group of soybean isoflavone glycosides, these methods There are disadvantages such as low yield, high conversion efficiency and low cost, harsh reaction conditions and harmful to the health of the body, so it cannot be widely applied

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This embodiment relates to a method for increasing the conversion rate of soybean isoflavones by fermenting soybean meal feed with compound probiotics, said method comprising the following steps:

[0027] Select and crush 55 parts of 20-mesh soybean meal feed, 40 parts of water, and 5 parts of lactobacillus, mix them evenly, pack them into bags and seal them, and carry out fermentation and cultivation at 35°C for 72 hours to obtain the finished product.

[0028] In this embodiment, high performance liquid chromatography is used to detect the content of daidzin, daidzein, daidzein, glycitein, genistin and genistein, and the pH value and dry matter recovery rate before and after fermentation are measured with a pH meter. DPPH scavenging ability and hydroxyl radical scavenging ability in the feed before and after fermentation were detected with the kit.

[0029] Implementation effect: the conversion rate of soybean isoflavone aglycone obtained by the method of this example...

Embodiment 2

[0031] This embodiment relates to a method for increasing the conversion rate of soybean isoflavones by fermenting soybean meal feed with compound probiotics, said method comprising the following steps:

[0032] Select and crush 53 parts of 50-mesh soybean meal feed, 40 parts of water, and 7 parts of Bacillus subtilis, mix them evenly, pack them into bags and seal them, and carry out fermentation and cultivation at 25°C for 360 hours to obtain the finished product.

[0033] In this embodiment, high performance liquid chromatography is used to detect the content of daidzin, daidzein, daidzein, glycitein, genistin and genistein, and the pH value and dry matter recovery rate before and after fermentation are measured with a pH meter. The scavenging ability of DPPH and hydroxyl radical of the feed before and after fermentation was detected with a kit.

[0034] Implementation effect: the conversion rate of soybean isoflavone aglycone obtained by the method in this example is 81.02%...

Embodiment 3

[0036] This embodiment relates to a method for increasing the conversion rate of soybean isoflavones by fermenting soybean meal feed with compound probiotics, said method comprising the following steps:

[0037] Select 62 parts of crushed 20-mesh soybean meal feed, 35 parts of water, and 3 parts of yeast, mix well, pack and seal, and carry out fermentation and cultivation at 40°C for 288 hours to obtain the finished product.

[0038] In this embodiment, high performance liquid chromatography is used to detect the content of daidzin, daidzein, daidzein, glycitein, genistin and genistein, and the pH value and dry matter recovery rate before and after fermentation are measured with a pH meter. The scavenging ability of DPPH and hydroxyl radical of the feed before and after fermentation was detected with a kit.

[0039] Implementation effect: the conversion rate of soybean isoflavone aglycone obtained in this example is 82.14%; the pH is 3.74; the dry matter recovery rate is 95.24...

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Abstract

The invention provides a method for improving soy isoflavone conversion rate of a composite probiotics fermented soybean meal feed. The method comprises the following steps: uniformly stirring crushed soybean meal, water and composite probiotics, sealing up and fermenting so as to obtain the feed. The method adopts a microorganism solid fermentation process, the used raw materials do not need to be sterilized, the fermentation process is simple, the production cost is low, and the soy isoflavone aglycone activity is high. The fermented soybean meal feed provided by the invention is thick in fragrance, capable of greatly improve the feed intake of animals, golden yellow and floppy in texture, and the activity of the soy isoflavone after fermentation is greatly improved. By adopting the process method, the recycling rate of dry substances is high.

Description

technical field [0001] The invention relates to a method for preparing soybean isoflavone aglycone in the technical field of animal husbandry, in particular to a method for improving the conversion rate of soybean isoflavones by fermenting soybean meal feed with compound probiotics. Background technique [0002] Soy isoflavones (SIF) are secondary metabolites formed during soybean growth. Mainly distributed in the seed coat, hypocotyl and cotyledon of soybean. So far, there are 12 isoflavone isomers known in soybean, which are divided into two types: free aglycone (Aglycon) and bound type glycoside (Glycosides). Aglycones account for 2 to 3% of the total, including genistein, daidzein and glycitein; glycosides account for 97 to 98% of the total, mainly genistein ( Genistin), daidzin (Daidzin), daidzin (Glycitin) and malonyl genistin, malonyl daidzin, malonyl daidzin exist in the form, accounting for about 95% of the total. [0003] SIF has a variety of biological activiti...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/00
Inventor 徐建雄杨守凤包健盛永帅
Owner SHANGHAI JIAO TONG UNIV
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