Method for improving various bionic functions on surface of cardiovascular implant material

Abstract

The invention discloses a method for improving various bionic functions on the surface of a cardiovascular implant material. The method comprises the following steps: first, preparing an HA (Hyaluronic Acid) stripe-like micrograph with high molecular weight, cultivating vascular endothelial cells on the surface, and regulating in vitro construction of extracellular matrixes of vascular endothelial cells through the HA stripe-like micrograph; and then, peeling the endothelial cells on the surface of the material by a decellularizing technology so as to prepare a multifunctional biological modified surface with anticoagulation / endothelialization promotion / smooth muscle cell adhesion inhibition effects and macrophage adhesion proliferation / inhibition and spreading on the surface of the cardiovascular implant material. The method disclosed by the invention constructs the multifunctional bionic endothelial cell extracellular matrix modified layer which is of anticoagulation and reendothelialization, inhibits adhesion and proliferation of smooth muscle cells, promotes phenotype shrink and inhibits adhesion and spreading of macrophage. The blood compatibility of the material, the structural and functional repair capacity of endothelial injury are remarkably improved.

Description

technical field [0001] The invention belongs to the intersection field of biological material surface engineering and biological imitation manufacturing engineering. Background technique [0002] The surface modification technology of cardiovascular implant materials has gradually matured and is widely used in clinical practice. However, the surface efficacy of materials currently used in clinical practice is far from reaching the expected goal. This is mainly due to the fact that no matter how good the surface modification is, its bionic function cannot match the sound biological function of the blood contact interface in the body's own cardiovascular system. [0003] Through the biomimicry of the surface of the material, endowing the material with good blood compatibility, biomimetic re-endothelialization ability, ability to inhibit the proliferation of smooth muscle cells and the ability to inhibit the adhesion of macrophages is the basis of the new biomimetic biochemica...

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Problems solved by technology

[0007] The preparation technology of HA micropatterns on the surface of biomaterials is a relatively mature existing technology, which can achieve the purpose of regulating cell morphology, behavior and function by preparing HA micropatterns of different sizes on the surface of materials, but the prepared surface blood phase Poor compatibility, its anticoagulant effect is not even as good as the original material surface; one or two components in the endothelial extracellular matrix are fixed on the surface of biomaterials to achieve the dual effects of anticoagulant and endothelial promotion. This is a mature existing technology, but the disadvantage is that the constructed surface only focuses on the biomimetic of one or two functions, rather than the biomimicry of the complete function of the inner wall of the blood vessel; the relatively complete endothelial extracellular matrix obtained by deendothelial cell technology on the surface of in vitro materials is currently also It has been reported, but the function of immune rejection and inhibition of inflammatory response of this surface has not been described. In addition, the culture of endothelial cells during the surface preparation process has not been carried out under biomimetic conditions. Its morphology, function and secreted extracellular matrix Whether it has complete functions has not been fully demonstrated

However, there is no report on obtaining this multifunctional biomimetic endothelial extracellular matrix by using striped HA micropatterns on the surface of cardiovascular materials to regulate endothelial cell fusion in vitro.

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