Mimic enzyme test paper for detecting hydrogen peroxide and application thereof

A technology of hydrogen peroxide and imitation enzyme, which is applied in the field of imitation enzyme test paper, can solve the problems of cumbersome test methods, etc., and achieve the effects of fast test speed, simple and feasible process, and simple structure

Active Publication Date: 2014-03-26
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although, currently H 2 o 2 There are many kinds of analysis methods, but the test methods are relatively cumbersome, and large and heavy instruments need to be carried for analysis during the test process. Therefore, for on-site testing, especially for long-distance testing, these methods have great problems. Therefore, the establishment of a fast and accurate H 2 o 2 The detection method is very important

Method used

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  • Mimic enzyme test paper for detecting hydrogen peroxide and application thereof
  • Mimic enzyme test paper for detecting hydrogen peroxide and application thereof
  • Mimic enzyme test paper for detecting hydrogen peroxide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Take 0.6g of filter paper or cotton, dissolve it into 60ml of ethanol solution added with 5% (mass percentage) TEOS and 2% KH-550 (purchased by Dow Corning, mass percentage), soak at room temperature for 0.5h, then suction filter, at 100℃ Keep the heat preservation for 10h to obtain SiO 2 coated cellulose (see figure 1 ), then the SiO 2 Coated fibers were immersed into 5wt% FeCl 3 The ethanol solution was refluxed for 24h, then removed and washed repeatedly with ethanol until Fe-free 3+ Outflow (can be tested with silver nitrate solution, the washing liquid has no white precipitate, and then dried at 100°C for 24 hours to obtain a cellulose hybrid material loaded with catalase; finally, the hybrid material was impregnated in 5wt% TMB containing in ethanol solution for 2 hours, and then dried at 100°C for 10 hours to obtain H 2 o 2 Imitation enzyme test strips for detection of imitation enzyme catalysts (see Figure 4b ).

[0038] To detect different H 2 o 2 Co...

Embodiment 2

[0040] Take 0.1g of filter paper or cotton, dissolve it into 40ml of ethanol solution with 5% (mass percentage) TEOS and 2% KH-580 (purchased by Dow Corning, mass percentage), impregnate at room temperature for 0.5h, then suction filter, at 100℃ Keep the heat preservation for 10h to obtain SiO 2 Coated cellulose, then SiO 2 Coated fibers were immersed into 5wt% FeCl 3 The ethanol solution was refluxed for 24h, then removed and washed repeatedly with ethanol until Fe-free 3+ outflow, and then dried at 100°C for 24h to obtain a cellulose hybrid material loaded with catalase; finally, the hybrid material was soaked in an ethanol solution containing 1wt% TMB for 2h, and then dried at 100°C for 10h to obtain h 2 o 2 Imitation enzyme test paper for detection of imitation enzyme catalyst.

[0041] To detect different H 2 o 2 Concentration, first with different concentrations of H 2 o 2 Solution (0.001mM-100mM), take 10ml of the solution to be tested during the test, immerse ...

Embodiment 3

[0043] Take 0.6g of filter paper or cotton, dissolve it into 60ml of acetol solution added with 5% (mass percentage) TEOS and 2% KH-550 (purchased by Dow Corning, mass percentage), impregnate at room temperature for 0.5h, then suction filter, and filter at 100 ℃ for 10 hours to obtain SiO 2 Coated cellulose, then SiO 2 Coated fibers were immersed into 5wt% FeCl 3 The ethanol solution was refluxed for 24h, then removed and washed repeatedly with ethanol until Fe-free 3+ outflow, and then dried at 100°C for 24 hours to obtain a cellulose hybrid material loaded with catalase; finally, the hybrid material was soaked in an ethanol solution containing 5wt% TMB for 2 hours, and then dried at 100°C for 10 hours to obtain h 2 o 2 Imitation enzyme test paper for detection of imitation enzyme catalyst.

[0044] To detect different H 2 o 2 Concentration, first with different concentrations of H 2 o 2 Solution (0.001mM-100mM), take 10ml of the solution to be tested during the test...

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Abstract

The invention relates to detection on hydrogen peroxide, and specifically discloses mimic enzyme test paper for detecting hydrogen peroxide, and preparation and application thereof. The mimic enzyme test paper is a surface modified cellulosic material loaded with an iron-based compound and a color developing agent, wherein the load amount of the iron-based compound is 1%-30% of the mass of the surface modified cellulosic material; the load amount of the color developing agent is 0.1%-20% of the mass of the surface modified cellulosic material; the color developing agent is 3, 3', 5, 5'-tetramethyl benzidine (TMB). According to the test paper provided by the invention, the color developing agent and the iron-based compound are loaded on the cellulosic material (such as filter paper, cotton and cotton cloth), the test paper simulates enzymatic decomposition of hydrogen peroxide and detects hydrogen peroxide, the mimic enzyme test paper is used in chemical industry, spinning, environment protection, medical treatment and food processing, and the application belongs to detection on hydrogen peroxide in the range of food safety, medical treatment, public health and environmental technology.

Description

technical field [0001] The invention relates to the detection of hydrogen peroxide, in particular to an enzyme imitation test paper for detecting hydrogen peroxide and its preparation and application. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) is an important chemical substance in the biological system, it seriously affects the cell function and metabolism, high concentration of H 2 o 2 even cause cell death. It is also accompanied by H during many enzymatic reactions, protein accumulation and antigen-antibody recognition 2 o 2 production or consumption. h 2 o 2 The detection of detection is also of great significance in the environment, food and other fields. Foreign developed countries have adopted H 2 o 2 For environmental protection, its effective application and outstanding effect are still highly respected by environmental workers and safety workers, and its application scale is also showing a growing trend. However, due to cost issues, there...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 姜义军牟新东李秀涛赵富华周剑伟
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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