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Method for rapidly determining caffeine in drink

A rapid determination and caffeine technology, applied in the direction of measurement device, material separation, analysis material, etc., can solve the problems of low analysis efficiency, high analysis cost, and high operation intensity.

Inactive Publication Date: 2014-03-26
GUANGXI UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for the rapid determination of caffeine in beverages, aiming to solve the problems of low analysis efficiency, high operation intensity and high analysis cost of existing caffeine detection methods in beverages

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  • Method for rapidly determining caffeine in drink
  • Method for rapidly determining caffeine in drink
  • Method for rapidly determining caffeine in drink

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Embodiment Construction

[0037] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0038] A method for the rapid determination of caffeine in beverages, such as figure 1 shown, including the following specific steps:

[0039] S1. Collect beverage samples and multi-wavelength chromatographic data of caffeine standard solution

[0040] In step S1, more specifically, the following steps are included:

[0041] S1-A, processing of beverage samples

[0042] After ultrasonically degassing commercially available Coca-Cola and Pepsi for 20 minutes, dilute 10-20 times; functional Red Bull beverage does not need to be degassed, dilute 10-20 times.

[0043] S1-B, establishment of backgro...

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Abstract

The invention provides a method for rapidly determining caffeine in a drink. The method comprises the steps as follows: acquiring multi-wavelength chromatographic data of drink samples and a caffeine standard solution; deducting data with the peak retention time identical with the caffeine standard solution from the chromatographic data of the drink samples; combining a plurality of drink samples and deducting chromatographic data of to-be-determined components, obtaining a background spectrum database containing no caffeine component, and calculating the amount of main components; reducing the dimension of the background database through singular value decomposition in combination with the amount of the main components to obtain a background database with a smaller data size; and calculating the content of the to-be-determined components in the drink samples by using space included angle criteria so as to realize quantification of the caffeine in the drink samples. The method is high in analysis efficiency, low in operation intensity, low in analysis cost, capable of reducing machine hours and reagent loss, good in robustness, simple and quite suitable for rapid assay determination of mass similar samples.

Description

technical field [0001] The invention belongs to the technical field of caffeine content determination, in particular to a method for rapidly determining caffeine in beverages. Background technique [0002] Caffeine, also known as caffeine, has a chemical name of 1,3,7-trimethylxanthine. It is soluble in water, alcohol, chloroform, dichloromethane and other solvents. It has functions such as promoting blood circulation, eliminating fatigue, and stimulating the central nervous system. Excessive addition can also cause ischemic heart disease, resulting in arrhythmia. Caffeine is widely added to functional Red Bull drinks and cola drinks such as Coca-Cola and Pepsi, so the caffeine content is also an important indicator to measure the quality of drinks. There are many methods for the determination of caffeine in food, such as centrifugal extraction-ultraviolet spectroscopy, ultraviolet second derivative spectroscopy, thin-layer chromatography and high performance liquid chromat...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/86
Inventor 粟晖刘柳姚志湘陈成孙增强方凤
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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