Method for low-temperature extraction of human serum albumin employing ethanol
A technology of human albumin and low-temperature ethanol, which is applied in the field of preparing human albumin, can solve the problems that the albumin production yield needs to be improved, the precipitation of extracted components is not refined enough, the component separation period is long, and the like, so as to shorten the separation time. Period, beneficial to comprehensive utilization, mild effect of mixed reaction
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Embodiment 1
[0024] see figure 1 , the method for extracting human serum albumin with low-temperature ethanol, using human plasma as raw material, adopting low-temperature ethanol method and pressure filtration method to produce human serum albumin, the production operating pressure is less than 0.25MPa, comprising the following steps:
[0025] Step 1. Isolation of FI from human plasma:
[0026] Control the temperature of the fused human plasma at 0.0°C, adjust the protein concentration to 40g / L, adjust the solution conductivity to 12.5ms / cm, pH value to 6.80, ethanol concentration to 7.0% by volume, and control the temperature to -1.0°C After the precipitation is complete, use a filter press to filter under pressure, choose a deep layer filter plate with a pore size of no more than 0.9 μm (such as 0.9 μm), control the temperature of the filter press to below -1°C, and filter to separate FI was precipitated to obtain FI supernatant;
[0027] Step 2. Separation of FⅡ+Ⅲ from the supernatan...
Embodiment 2
[0037] see figure 1 , the method for extracting human serum albumin with low-temperature ethanol, using human plasma as raw material, adopting low-temperature ethanol method and pressure filtration method to produce human serum albumin, the production operating pressure is less than 0.25Mpa, including the following steps:
[0038] Step 1. Isolation of FI from human plasma:
[0039]Control the temperature of the fused human plasma between 2.0°C, adjust the protein concentration to 52.5g / L, adjust the solution conductivity to 13.1ms / cm, pH to 7.05, ethanol concentration to 8.5% by volume, and control the temperature to -2.0°C, after the precipitation is complete, use a filter press to filter under pressure, choose a deep layer filter plate with a pore size of no more than 0.9 μm (such as 0.6 μm), and control the temperature of the filter press to below -1°C. The FI precipitate was separated by filtration to obtain the FI supernatant;
[0040] Step 2. Separation of FⅡ+Ⅲ from th...
Embodiment 3
[0050] see figure 1 , the method for extracting human serum albumin with low-temperature ethanol, using human plasma as raw material, adopting low-temperature ethanol method and pressure filtration method to produce human serum albumin, the production operating pressure is less than 0.25Mpa, comprising the following steps:
[0051] Step 1. Isolation of FI from human plasma:
[0052] Control the temperature of the fused human plasma at 4.0°C, adjust the protein concentration to 65g / L, adjust the conductivity of the solution solution to 13.7ms / cm, adjust the pH value to 7.30, adjust the ethanol concentration to 10.0% by volume, and control the temperature to - 3.0°C, after the precipitation is complete, use a filter press to filter under pressure, choose a deep layer filter plate with a pore size of no more than 0.9 μm (such as 0.3 μm), control the temperature of the filter press to below -1°C, and filter Separate the FI precipitate to obtain the FI supernatant;
[0053] Step ...
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