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Preparation method of eucommia seed protein

A technology of seed protein and seed meal, which is applied in the field of protein extraction, can solve the problems of waste of resources, insufficient development and utilization of Eucommia seeds, and high added value of products, and achieve the effect of ensuring quality, protein extraction rate, and high product yield

Inactive Publication Date: 2014-04-16
JISHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problems of insufficient development and utilization of eucommia seeds and the waste of resources, the inventor finally invented a method for extracting protein from eucommia seeds after a large number of production practices. The method has short extraction time, high efficiency and simple operation , the biologically active ingredients are well preserved, and the resulting products have high added value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Treatment of eucommia seeds: dry the eucommia seeds and crush them to 30 meshes, put them into a supercritical carbon dioxide extraction kettle, remove the fat from the eucommia seeds through supercritical carbon dioxide extraction, and obtain the eucommia seed meal for later use.

[0014] (2) Extraction and separation of eucommia seed protein: add eucommia ulmoides seed meal to 12 times its mass of water, then add sodium hydroxide solution to adjust the pH value to 9.5, put it in the microwave and turn on the microwave to make the solution boil for 4.5 minutes, and centrifugally filter And collecting the obtained filtrate, the filtrate is the eucommia seed protein solution, adding hydrochloric acid to the filtrate to adjust the pH value to 3.8 to precipitate the protein, and freeze-drying the obtained precipitate to obtain the eucommia seed protein.

[0015] The eucommia seed protein obtained by the above method has a mass fraction of protein of 71% and aucubin of 7...

Embodiment 2

[0017] (1) Treatment of eucommia seeds: dry the eucommia seeds and crush them to 50 meshes, put them into a supercritical carbon dioxide extraction kettle, remove the fat from the eucommia seeds through supercritical carbon dioxide extraction, and obtain the eucommia seed meal for later use.

[0018] (2) Extraction and separation of eucommia seed protein: add eucommia seed meal to 20 times its mass of water, then add sodium hydroxide solution to adjust the pH value to 11.2, put it in the microwave and turn on the microwave to make the solution boil for 7 minutes, and centrifugally filter And collecting the obtained filtrate, the filtrate is the eucommia seed protein solution, adding hydrochloric acid to the filtrate to adjust the pH value to 3.2 to precipitate the protein, and freeze-drying the obtained precipitate to obtain the eucommia seed protein.

[0019] The eucommia seed protein obtained by the above method has a mass fraction of protein of 62% and aucubin of 13.6%.

Embodiment 3

[0021] (1) Treatment of eucommia seeds: dry the eucommia seeds and crush them to 70 meshes, put them into a supercritical carbon dioxide extraction kettle, remove the fat from the eucommia seeds through supercritical carbon dioxide extraction, and obtain eucommia seed meal for later use.

[0022] (2) Extraction and separation of eucommia seed protein: add eucommia seed meal to 28 times its mass of water, then add sodium hydroxide solution to adjust the pH value to 8.4, put it in the microwave and turn on the microwave to make the solution boil for 9.5 minutes, and centrifugally filter The obtained filtrate is collected, and the filtrate is the eucommia seed protein solution. Hydrochloric acid is added to the filtrate to adjust the pH value to 4.6 to precipitate the protein, and the obtained precipitate is freeze-dried to obtain the eucommia seed protein.

[0023] The eucommia seed protein obtained by the above method has a mass fraction of protein of 79% and aucubin of 3.8%.

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Abstract

The invention discloses a preparation method of eucommia seed protein. The method comprises the following steps: (1) drying and crushing eucommia seeds to 20-80 meshes, putting the crushed eucommia seeds in a supercritical carbon dioxide extraction kettle, and removing grease in the eucommia seeds by supercritical carbon dioxide extraction to obtain eucommia seeds for later use; and (2) adding water in the eucommia seeds, regulating the pH value to 8-12, starting microwave to boil the solution for 3-10 minutes, centrifugally filtering and collecting filtrate, adding hydrochloric acid in the filtrate to regulate the pH value to 3-5 so as to precipitate protein, and carrying out freeze drying to obtain the eucommia seed protein. The preparation method disclosed by the invention has the advantages of high extraction efficiency, simple operation, high product yield and good storage of active ingredient aucubin, and the extraction rate of protein in the eucommia seeds is higher than 80%.

Description

technical field [0001] The invention relates to a method for extracting protein from eucommia seeds, in particular to a method for preparing eucommia seed protein by adopting supercritical carbon dioxide degreasing and microwave-assisted alkali extraction. Background technique [0002] Eucommia is a unique economic tree species in my country. It is mainly distributed in Guizhou, Sichuan, Hunan, Hubei and other provinces south of the Qinling Mountains. The planting area is more than 350,000 hectares. Eucommia can bloom and set seeds every year. resource. Eucommia ulmoides seeds contain about 28-35% protein, which is an ideal raw material for extracting protein. In addition, eucommia ulmoides seeds also contain oil, aucubin, etc. Among them, aucubin is an important biologically active substance, which has the effect of clearing away dampness and heat. , urination, analgesia, blood pressure lowering, liver protection, anti-tumor and other important health effects. [0003] At ...

Claims

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Application Information

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IPC IPC(8): A23J1/14
Inventor 黄群杨万根麻成金王珏揭雅娇杨志伟
Owner JISHOU UNIVERSITY
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