Vegetable oil body gel containing EGF (Epidermal Growth Factor)

A vegetable oil body and gel technology, applied in the biological field, can solve problems such as inconvenient use
CN103736079AInactive Publication Date: 2014-04-23JILIN AGRICULTURAL UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JILIN AGRICULTURAL UNIV
Publication Date
2014-04-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a vegetable oil body gel containing EGF (Epidermal Growth Factor). The vegetable oil body gel is obtained by mixing a vegetable oil body containing the EGF and a gel matrix of the oil body, wherein the gel matrix of the oil body is composed of 1%-5% of carboxyethyl cellulose, 5%-40% of glycerol, 0.01%-0.1% of methyl paraben, 0.01%-0.1% of propyl paraben, and water injection added to 100%. The vegetable oil body containing the EGF is obtained through the steps of connecting the nucleotide sequence of human EGF to the terminal 3' of the oil body protein nucleotide sequence of the oil body expression vector p1390ONE, transferring to the oil plant so that the EGF is expressed in the oil body, and then extracting the oil body. The prepared vegetable oil body gel is free of mildew and the physicochemical indexes such as appearance and pH of the vegetable oil body gel all are not changed after the vegetable oil body gel is preserved for 12 months at 37 DEG C; even though the vegetable oil body gel is preserved for 12 months at 4 DEG C, the activity of the EGF has no obvious change.
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Description

technical field

[0001] The invention relates to the field of biological technology, and specifically relates to vegetable oil bodies containing EGF and vegetable oil body gels containing EGF. Background technique

[0002] The oil body is composed of a single layer of phospholipid membrane, which contains TAG, and the surface is inlaid with oil body protein, calcium binding protein and sterol binding protein. TAG stored in oil bodies provides energy for seed germination. Oleosins are the major membrane proteins in oil bodies. It is a basic hydrophobin composed of three parts: the lipophilic N-terminus and C-terminus, and the intermediate hydrophobic region. The N-terminus and C-terminus are inlaid on the surface of the oil body and exposed in the cytoplasm. The hydrophobic region in the middle enters the interior of the oil body through the phospholipid membrane. So it creates electrical resistance and electrostatic repulsion, allowing the oil body to exist independently....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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