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Method for reducing crystallinity of bacterial cellulose in fermentation process

A technology for bacterial cellulose and fermentation process, applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of cellulose molecular weight loss, cost increase, affecting cellulose mechanical properties, etc. Good control effect, simple and fast effect

Active Publication Date: 2014-04-23
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology usually uses the method of acid and alkali to partially degrade the synthesized bacterial cellulose to obtain the required crystallinity, but this method is easy to cause the loss of the molecular weight of the cellulose, thus affecting the mechanical properties of the cellulose when it is used; now Some technologies also change the shape of bacterial cells by adding some polysaccharides or drugs to obtain bacterial cellulose with different crystallinity, but it needs to add more polysaccharides or drugs (the volume fraction added is 2% to 10%), and the cost will also increase. corresponding increase

Method used

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  • Method for reducing crystallinity of bacterial cellulose in fermentation process
  • Method for reducing crystallinity of bacterial cellulose in fermentation process
  • Method for reducing crystallinity of bacterial cellulose in fermentation process

Examples

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Embodiment 1

[0039] A method for reducing the crystallinity of bacterial cellulose in a fermentation process, comprising the following steps:

[0040] (1) Prepare the culture medium, the composition of the culture medium is: 0.2g / mL glucose, 0.02g / mL peptone, 0.02g / mL yeast powder, 0.005g / mL disodium hydrogen phosphate, 0.001g / mL magnesium sulfate, 0.01g / mL ammonium sulfate, 0.005mL / mL corn syrup extract;

[0041] (2) Inoculate 1 mL of Acetobacter xylinum with the preservation number ATCC700178 into 100 mL of the above-mentioned culture solution; filter and sterilize the fluorescent whitening agent through a 25 μm filter membrane, and then add 0.01 mL of fluorescent whitening agent to the culture solution The agent Fluka18909 (purchased from Simga) was rotated and fermented at 125rpm for 120h. After the fermentation, the culture solution was poured out and the generated cellulose was rinsed with deionized water until no residue of the culture solution remained. After steam sterilization, t...

Embodiment 2

[0043]A method for reducing the crystallinity of bacterial cellulose in a fermentation process, comprising the following steps:

[0044] (1) Prepare the culture medium, the composition of the culture medium is: 0.2g / mL glucose, 0.02g / mL peptone, 0.02g / mL yeast powder, 0.005g / mL disodium hydrogen phosphate, 0.001g / mL magnesium sulfate, 0.01g / mL ammonium sulfate, 0.005mL / mL corn syrup extract;

[0045] (2) Inoculate 1mL of Acetobacter xylinum with preservation number ATCC700178 into 100mL of the above-mentioned culture solution; filter and sterilize the fluorescent whitening agent through a 25μm filter membrane, and then add 1mL of fluorescent whitening agent to the culture solution Fluka18909 (purchased from Simga), rotated and fermented at 125rpm for 120h. After the fermentation, pour out the culture solution and rinse the generated cellulose with deionized water until no residual solution of the culture solution remained. After steam sterilization, bacterial fibers were obtai...

Embodiment 3

[0050] A method for reducing the crystallinity of bacterial cellulose in a fermentation process, comprising the following steps:

[0051] (1) Prepare the culture medium, the composition of the culture medium is: 0.2g / mL glucose, 0.02g / mL peptone, 0.02g / mL yeast powder, 0.005g / mL disodium hydrogen phosphate, 0.001g / mL magnesium sulfate, 0.01g / mL ammonium sulfate, 0.005mL / mL corn syrup extract;

[0052] (2) Inoculate 1mL of Acetobacter xylinum solution with preservation number ATCC700178 into 100mL of the above culture solution; filter and sterilize the fluorescent whitening agent through a 25μm filter membrane, and then add 1mL of fluorescent whitening agent Fluka18909 to the culture solution (purchased from Simga), cultured and fermented statically at 28°C for 120 hours. After the fermentation, pour out the culture solution and rinse the generated cellulose with deionized water until no residual solution of the culture solution remained. After steam sterilization, bacteria wer...

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Abstract

The invention provides a method for reducing the crystallinity of bacterial cellulose in a fermentation process. The method comprises the steps of preparing a culture solution, inoculating a bacterial cellulose producing strain into the culture solution, then performing static fermentation under 28-32 DEG C or performing rotary fermentation at 100-200 rpm for 100-120 hours, adding a fluorescent whitening agent into the culture solution in the initial stage in the fermentation process, and pouring out the culture solution at the end of fermentation to obtain the bacterial cellulose, wherein the fluorescent whitening agent is 0.01-1 percent of the culture solution by volume. According to the method, the fluorescent whitening agent is added in the fermentation process of bacteria, so that the aggregation of a fiber band of the bacterial cellulose is alleviated, and the crystallinity of the bacterial cellulose is lowered. The method is simple and quick, so that the molecular weight of the cellulose cannot be affected, and the cost is low; the crystallinity of the bacterial cellulose can be well regulated and controlled.

Description

technical field [0001] The invention relates to a method for reducing the crystallinity of bacterial cellulose, in particular to a method for reducing the crystallinity of bacterial cellulose in a fermentation process. Background technique [0002] Cellulose, a biopolymer with β-1,4 glycosidic glucose units, is the most widespread source of biomass on Earth. Different from plant cellulose, bacterial cellulose is an exopolysaccharide secreted by bacteria. Bacterial cellulose usually has high purity (more than 95% cellulose content), high crystallinity (more than 80% crystallinity), nanostructure (microfibrous structure of tens of nanometers) and high mechanical strength, it is widely used in food, papermaking and textile fields. [0003] However, a large number of hydrogen bonds are formed between bacterial cellulose and between bacterial cellulose and water molecules. These hydrogen bonds form a huge hydrogen bond network, which directly leads to the formation of a dense cr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02
Inventor 胡阳周新王金慧朱勇军周阳潘浩波
Owner SHENZHEN INST OF ADVANCED TECH