Cryopreservation liquid for equine animal embryo, and preparation method and application thereof

A cryopreservation solution and embryo technology, applied in the field of animal embryo transplantation, can solve the problems of cumbersome preparation steps, high price, and difficult drug sources, etc., and achieve the effects of simple preparation, convenient material collection, and favorable promotion and application

Inactive Publication Date: 2014-04-30
QINGDAO DERUI JUNFA BIOLOGY TECH +2
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of traditional embryo cryopreservation solutions such as difficult source of drugs, high price, cumbersome preparation steps, and the need to use and prepare immediately, the present invention provides a non-surgical embryo freezing method suitable for large-scale production of equine animal embryo transfer. Preservative solution and its preparation method and application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] An equine embryo cryopreservation solution comprises 5000ml of sodium lactate mixed solution as the main solution, and every 1000ml of sodium lactate mixed solution contains 500,000 units of penicillin, 500,000 units of streptomycin and 10% by volume of calf serum.

[0022] Weigh 27g of sodium lactate, 16g of sodium chloride, 23g of potassium chloride, 5.5g of calcium chloride and 1.6g of magnesium chloride, add 5000ml of distilled water to mix, place in a thermostat at 37°C for 6 hours, and set aside; weigh 0.4g of astragalus polysaccharide Put it into a sterile beaker, add it to the spare mixed solution into the sterile beaker, stir to fully dissolve the astragalus polysaccharide, and obtain a sodium lactate mixed solution; before cryopreservation of the embryos, add penicillin, Streptomycin and calf serum can be used to obtain embryo cryopreservation solution.

[0023] Use the embryo cryopreservation solution of this embodiment to cryopreserve non-surgical bovine emb...

Embodiment 2

[0025] An equine animal embryo cryopreservation solution includes 5000ml of sodium lactate mixed solution as the main solution, and every 1000ml of sodium lactate Ringer injection contains 1 million units of penicillin, 1.2 million units of streptomycin and 3% inactivated horse serum by volume.

[0026] Weigh 10.5g of sodium lactate, 6g of sodium chloride, 13.5g of potassium chloride and 9g of calcium chloride, add 5000ml of distilled water to mix, place in a thermostat at 37°C for 3 hours, and set aside; weigh 1.4g of astragalus polysaccharide into Add it to the sterile beaker, add it to the spare mixed solution, stir to fully dissolve the astragalus polysaccharide, and get the sodium lactate mixed solution; before cryopreservation of the embryo, add penicillin and streptomycin to the sodium lactate mixed solution according to the component content And inactivated horse serum, the embryo cryopreservation solution can be obtained.

[0027] The cryopreservation solution for emb...

Embodiment 3

[0029] An equine animal embryo cryopreservation solution, including 4000ml of sodium lactate mixture as the main solution, wherein every 1000ml of sodium lactate mixture contains 800,000 units of penicillin, 800,000 units of streptomycin and 5% inactivated horse serum by volume; Wherein, the content of each component in the sodium lactate mixed solution is: every 1000ml sodium lactate mixed solution contains 26g of sodium lactate, 10.8g of sodium chloride, 21g of potassium chloride and 16.8g of calcium chloride.

[0030] Weigh 26g of sodium lactate, 10.8g of sodium chloride, 21g of potassium chloride and 16.8g of calcium chloride, add 4000ml of distilled water to mix, and place in a constant temperature box at 37°C for 1 hour to obtain a sodium lactate mixture for later use; cryopreservation of embryos Before, add penicillin, streptomycin and inactivated horse serum to the sodium lactate mixture according to the content of the components to obtain the embryo cryopreservation so...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a cutting operation liquid for equine animal embryos, and a preparation method and application thereof. The cutting operation liquid includes a sodium lactate mixed solution, penicillin, streptomycin, and one, two or three selected from bovine serum albumin, calf serum and inactivated equine serum. The preparation method includes the steps of: placing the sodium lactate mixed solution in a thermostat at 37 DEG C for 0.5-6 h for standby; and adding penicillin, streptomycin and one, two or three selected from bovine serum albumin, calf serum and inactivated equine serum into the sodium lactate mixed solution in accordance with the component contents, so as to obtain the cutting operation liquid for embryos. The cutting operation liquid is suitable for non-surgical method for embryo transplantation of horses, cattle and donkeys. The invention has the beneficial effect of convenience for material acquisition, low price, simple preparation and convenience for usage, is very suitable for scale production of non-surgical method for embryo transplantation of equine animals, and is favorable for popularization and application of embryo transplantation technology.

Description

technical field [0001] The invention belongs to the field of animal embryo transplantation, in particular to an equine animal cryopreservation solution and its preparation method and application. Background technique [0002] Put the embryos and freezing liquid into the freezing tube, and through slow (2-3 day embryos) and fast (5-6 day blastocyst) cooling methods, the embryos can be stilled and can be stored at -196 degrees A method of preservation in liquid nitrogen. In the process of embryo transfer, if there are remaining embryos with good quality, they can be frozen and stored, and then implanted into the uterine cavity after natural cycle or artificial cycle after thawing, which will increase the chance of conception. [0003] Embryo cryopreservation solution is a protective nutrient solution for embryos during the freezing process. At present, there are few reports on the preservation of embryo cryopreservation solution at home and abroad. Especially in China, the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 邹敬清荣美洁邹志钢
Owner QINGDAO DERUI JUNFA BIOLOGY TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap