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Inactivated bacteroid micro-ecological preparation and preparation method thereof

A technology of micro-ecological preparations and inactivated Bacteroides, which is applied in food preparation, food science, animal feed, etc., can solve the problems of short shelf life, rareness, and inability to colonize for a long time, and achieve a stable effect

Inactive Publication Date: 2014-04-30
SUZHOU WANSHENGYUAN BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned compound probiotics can adjust the intestinal balance of humans or animals to a certain extent, thereby improving immunity, the above-mentioned probiotics has the following disadvantages: 1. The above-mentioned live bacteria preparations are easily affected by environmental conditions. It is easy to be inactivated during transportation and transportation, and the shelf life is short; 2. When the probiotics are used together with feed, the live bacteria are easily killed by the antibacterial substances added in the feed; 3. The live bacteria in the preparation enter the digestive tract Afterwards, it cannot effectively resist hydrochloric acid and bile salts, cannot reach the intestinal tract or cannot colonize the intestinal tract for a long time to function
However, research on inactivated probiotics is relatively rare in the prior art, and currently only lactic acid bacteria are reported, but inactivated probiotics are becoming an important direction of probiotics due to their unique advantages

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] An inactivated Bacteroides microecological preparation, containing in every kilogram of preparation: 450 grams of inactivated Bacteroides polymorpha and its fermentation product, wherein the content of inactivated Bacteroides polymorpha cells is 10 8 ~10 9 Each / gram, specification is 80 mesh corncob powder and precipitated silica 550 grams altogether, wherein each 225 grams of corncob powder and precipitated silica.

[0029] The preparation method of inactivated Bacteroides probiotics comprises the following steps:

[0030] S1. Fermentation of Bacteroides polymorpha: inoculate the fermentation medium with Bacteroides polymorpha with a seed age of 15 hours at an inoculum of 8% (v / v or v / w), and ferment at a temperature of 37°C , anaerobic culture for 48 hours; the fermentation medium is improved broth medium: improved broth medium pH 7.0±0.2, autoclaved at 121°C for 15 minutes, when cooled to about 50°C, add filter-sterilized vitamin K1 solution 0.5ml and hemin solutio...

Embodiment 2

[0036] An inactivated Bacteroides fragilis microecological preparation, containing 350 grams of inactivated Bacteroides fragilis and its fermentation product per kilogram of preparation, wherein the content of inactivated Bacteroides fragilis cells is 10 8 ~10 9 Each gram, cornstarch and precipitated silicon dioxide are 650 grams altogether, wherein cornstarch and precipitated silicon dioxide are each 325 grams.

[0037] The preparation method of described probiotics, comprises the steps:

[0038] S1. Fermentation of Bacteroides fragilis: Inoculate the fermentation medium with 12-hour-old Bacteroides fragilis at an inoculum size of 5% (v / v or v / w), and anaerobically Cultivate for 40 hours; the fermentation medium is improved broth medium: improved broth medium pH7.0±0.2, autoclaved at 121°C for 15 minutes, when cooled to about 50°C, add filter-sterilized vitamin K1 solution 0.5ml and 2.5ml of hemin solution (5mg / ml), mix well and set aside.

[0039] S2. After the fermentati...

Embodiment 3

[0044] An inactivated Bacteroides microecological preparation, containing 480 grams of inactivated Bacteroides fragilis and Pleomorpha and their fermentation products per kilogram of preparation, wherein the total cell content of inactivated Bacteroides fragilis and Pleomorpha is 10 8 ~10 9 Each / g, specification is 80 mesh corncob powders and precipitated silicon dioxide 520 grams altogether, wherein each 260 grams of corncob powders and precipitated silicon dioxide.

[0045] The preparation method of inactivated Bacteroides probiotics as mentioned above: comprises the following steps:

[0046] S1. Separate fermentation culture of Bacteroides fragilis and Bacteroides polymorpha: Bacteroides fragilis and Bacteroides polymorpha with a seed age of 16 hours were respectively inoculated into the fermentation medium at an inoculation amount of 8% (v / v or v / w), Under the condition of fermentation temperature of 37°C, anaerobic culture was carried out for 48 hours; the fermentation m...

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Abstract

The invention relates to the field of microbiological preparations, and particularly discloses an inactivated bacteroid micro-ecological preparation and a preparation method of the inactivated bacteroid micro-ecological preparation. The preparation uses inactivated bacteroid and fermentation concentrate as the main ingredients; and the inactivated bacterioid micro-ecological preparation has the efficacies of resisting bacteria, diminishing inflammation, adjusting the microbial community of the gastrointestinal tract, preventing gastrointestinal diseases of human and animals and the like, and has the characteristics of quick operation, high safety, use convenience and the like. The inactivated bacteroid micro-ecological preparation is used for disease prevention and health of human and animals, has a good practical application value, and has a remarkable effect especially when being applied to animal feeds, so that the probiotic inactivated micro-ecological preparation is a technology worth of population.

Description

technical field [0001] The invention relates to the field of probiotics, in particular to an inactivated Bacteroides probiotics and a preparation method thereof. Background technique [0002] Bacteroides are a large number of normal flora in humans and animals, accounting for more than 1 / 4 of the intestinal flora, and maintain a special environment for the intestinal tract with other intestinal flora and the host, which plays a significant role in the health of the host to an important role. Bacteroides can help the host decompose polysaccharides, form intestinal mucosal blood vessels, improve host immunity, and maintain intestinal microecological balance. There are many types of Bacteroides in the intestinal tract, which together constitute the intestinal Bacteroides flora, coordinate and complement each other to maintain the balance of the intestinal flora. Therefore, the effect of preparing a compound Bacteroides probiotics will be more significant than that of a single...

Claims

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Application Information

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IPC IPC(8): A23L1/30A23L2/52A23K1/16
CPCA23K20/00A23L2/52A23L33/135A23V2002/00
Inventor 胡文锋庞旭胡学华胡学生胡学锋潘海朋
Owner SUZHOU WANSHENGYUAN BIOLOGICAL SCI & TECH CO LTD
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