Preparation method and application of antimicrobial and anti-degradable small instestinal mucous accellular substrate

Abstract

The invention discloses a preparation method of an antimicrobial and anti-degradable small instestinal mucous accellular substrate. The preparation method comprises the following steps of 1) carrying out crosslinking on soaking genipin and small instestinal mucous accellular substrate, and then rinsing and drying; 2) transferring the small instestinal mucous accellular substrate which is crosslinked with genipin in the step 1) into a dopamine solution to be soaked, and then rinsing and drying; and 3) transferring the small instestinal mucous accellular substrate processed in the step 2) into a silver nitrate solution to be soaked, and then rinsing and drying. The invention also discloses application of the antimicrobial and anti-degradable small instestinal mucous accellular substrate in tissue repair of a high stress part. According to the preparation method, the small instestinal mucous accellular substrate is modified in antimicrobial and anti-degradable performances; the small instestinal mucous accellular substrate prepared by using the method has the antimicrobial and anti-degradable performances superior to those of a natural small instestinal mucous accellular substrate and also remains high biocompatibility, and thus the small instestinal mucous accellular substrate can be applied to the issue repair of the high-stress part.

Description

technical field [0001] The invention belongs to the technical field of tissue repair materials, and relates to a preparation method and application of an antibacterial and anti-degradation porcine small intestinal mucosa decellularized matrix. Background technique [0002] Natural biomaterial porcine intestinal mucosal acellular matrix is ​​currently the most widely used xenogeneic biomaterial in clinical practice, because it has a similar composition and structure to human extracellular matrix, and the main immunogenic components have been removed by decellularization technology, so biological It has good compatibility, low immunogenicity, and can better regulate cell adhesion, migration, proliferation and differentiation. It is an ideal scaffold material for tissue regeneration and repair. However, the existing porcine intestinal mucosal acellular matrix has the following two defects, which limit its further use in the field of regenerative medicine: [0003] First, the ...

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Problems solved by technology

However, the existing porcine intestinal mucosal acellular matrix has the following two defects, which limit its further use in the field of regenerative medicine:

[0003] First, the natural acellular matrix of porcine small intestinal mucosa degrades quickly. In some high-stress areas, such as in hernia repair and gynecological pelvic floor repair, the new matrix material is not yet fully mature and the implanted porcine small intestinal mucosa degrades rapidly. The cell matrix degrades, resulting in a decrease in biomechanical properties, so it cannot provide sufficient mechanical support for use in high-stress areas such as the pelvic floor

[0004] Second, the natural porcine small intestinal mucosal acellular matrix itself does not have active antibacterial properties, and infection related to material implantation is an important clinical complication at present, often leading to the need for reoperation

However, due to the mixed method, the combination of nano-silver and porcine small intestinal mucosal acellular matrix is ​​not tight enough, and a large amount of nano-silver will be released in a short period of time, so that the antibacterial effect in the later stage is limited, and further improvement is needed

[0007] In general, although there are certain preliminary studies on the anti-degradation and antibacterial modification of porcine small intestinal mucosal acellular matrix, there is no research on the simultaneous anti-degradation and antibacterial modification of porcine small intestinal mucosal acellular matrix. In particular, there is a lack of data on the reaction characteristics of the modified material at high stress sites (this is the main indicator for evaluating whether the modified material has application value)

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