A kind of simple preparation method and application of organic-inorganic composite liposome cerasome
A compound lipid and inorganic technology, applied in the chemical field, can solve the problems of long hydrolysis time and poor operability of compounds, and achieve the effects of shortened hydrolysis time, complete hydrolysis and strong operability
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Embodiment 1
[0044] A kind of flow chart of the preparation method of organic-inorganic composite liposome Cerasome such as figure 1 ,Specifically:
[0045] (1) Lipid (EtO) 3 SiC 3 Suc2C 16 Accurately weigh 15.38mg, add 6.84μL of ultrapure water, 3.00μL of 0.2mol / L HCl solution, 233.26μL of anhydrous ethanol, molar ratio [1] , to formulate synthetic lipids (EtO) 3 SiC 3 Suc2C 16 :H 2 The mixed solution of O:HCl:EtOH=1:19:0.03:200 was 243.1 μL, stirred at room temperature for 1 h, and the hydrolyzed solution was blown dry by nitrogen flow to obtain 12.57 mg of hydrolyzed product;
[0046] (2) After the hydrolyzed product was vacuum-dried at 35°C for 2 hours, a small amount of chloroform was added to completely dissolve the hydrolyzed product, and the chloroform was completely volatilized under nitrogen flow to form a film;
[0047] (3) The film was vacuum-dried at 35°C to completely remove residual chloroform, 20 mL of 10 mmol / L HEPES buffer solution (pH=7.2) was added, and vortexed...
Embodiment 2
[0055] Preparation of intercalated functional molecule 4-hexadecyloxy-2-hydroxybenzaldehyde thiosemicarbazide (see structure in Image 6 A) Organic-inorganic complex liposomal Cerasome.
[0056] Because such functional molecules are not easily soluble in ethanol, or are unstable and decompose during acid hydrolysis, they cannot be embedded in the liposome membrane without using the method of the present invention, thereby hindering the function of Cerasome to a certain extent. development and application, and this method can solve this problem, specifically:
[0057] The other steps are the same as those in Example 1, except that in step (2), the hydrolyzed product is vacuum-dried at 35-40 °C for 2 hours, and then 0.9 mg of functional molecule (4-hexadecyloxy-2-hydroxybenzaldehyde condensate) is added. Thiosemicarbazide), then add a small amount of chloroform to completely dissolve the hydrolyzate and the functional molecule, and make the chloroform completely volatilized und...
Embodiment 3
[0060] Liposomes were prepared by traditional methods. In order to explore whether the precursor compounds were completely hydrolyzed by the traditional method, the hydrolyzed solution was changed to deuterated reagents, which was convenient for sampling and NMR detection. The ultrapure water was changed to D. 2 O (heavy water), 0.2mol / L HCl solution was changed to 0.2mol / LDCl (deuterated hydrochloric acid) heavy water solution, anhydrous ethanol was changed to CD 3 OD (deuterated methanol), literature [2] It is reported that the hydrolysis effect of anhydrous ethanol is the same as that of anhydrous methanol. The preparation method is:
[0061] (1) Lipid (EtO) 3 SiC 3 Suc2C 16 Accurately weigh 15.38mg, add D 2 O6.84μL, 0.2mol / LDCl heavy aqueous solution 3.00μL, CD 3 OD233.26μL, molar ratio, formulated synthetic lipid (EtO) 3 SiC 3 Suc2C 16 :H 2 The mixed solution of O:HCl:EtOH=1:19:0.03:200 was 243.1 μL, stirred at room temperature, and samples were taken at regula...
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