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Method for preparing substrate for high formyl group functional group density biological chip

A high aldehyde functional group biochip technology, which is applied in the field of high aldehyde functional group density biochip substrates and its preparation, can solve the problems of large autofluorescence background and uneven surface of the substrate, and achieves the effect of solving uneven spotting

Active Publication Date: 2014-04-30
江苏贝思安特生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Purpose of the invention: The technical problem to be solved by the present invention is to propose a substrate for biochips with high aldehyde functional group density in view of the shortcomings of existing biochip substrates, such as large autofluorescence background after spotting, uneven substrate surface modification, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Blank glass slide processing technology: ultra-clear glass substrate selection 10 -6 -10 -7 m 3 Polish the aluminum oxide powder; insert it into the dyeing rack, put it into the ultrasonic cleaning machine, the water level is not over the dyeing rack, ultrasonically clean, wash for 10 minutes, centrifuge, and dry.

[0026] Amination treatment process: After polishing and cleaning, the blank sheet is placed in a vacuum oven, vacuumed, the vent hole is opened, and ultra-pure aminosilane is added. The temperature is set at 55 ° C, and the time is 35 minutes. It is used by vacuum evaporation. Substrate amination.

[0027] The preparation process of substrates for biochips with high aldehyde functional group density: put the amino sheets in a vacuum oven, vacuumize, open the vent holes, add ultra-pure adipaldehyde, set the temperature at 70°C, and use vacuum evaporation for 55 minutes. Aldehyde modified the substrate to obtain the aldehyde substrate.

[0028] Prepare a 1...

Embodiment 2

[0030] Blank glass slide processing technology: ultra-clear glass substrate selection 10 -8 -10 -9 m 3 Polish the aluminum oxide powder; insert it into the dyeing rack, put it into the ultrasonic cleaning machine, the water level is not over the dyeing rack, ultrasonically clean, wash for 10 minutes, centrifuge, and dry.

[0031] Amination treatment process: put the polished and cleaned blank sheet in a vacuum oven, vacuumize, open the vent hole, add ultra-pure aminosilane, set the temperature at 55°C, and use the vacuum evaporation method for 35 minutes. Substrate amination.

[0032] The preparation process of substrates for biochips with high aldehyde functional group density: put the amino sheets in a vacuum oven, vacuumize, open the vent holes, add ultra-pure adipaldehyde, set the temperature at 70°C, and use vacuum evaporation for 55 minutes. Aldehyde modified the substrate to obtain the aldehyde substrate.

[0033] Relevant testing steps are the same as in Example 1,...

Embodiment 3

[0035] Blank glass slide processing technology: ultra-clear glass substrate selection 10 -8 -10 -9 m 3 Polish the aluminum oxide powder; insert it into the dyeing rack, put it into the ultrasonic cleaning machine, the water level is not over the dyeing rack, ultrasonically clean, wash for 10 minutes, centrifuge, and dry.

[0036] Amination treatment process: put the polished and cleaned blank sheet in a vacuum oven, vacuumize, open the air hole, add ultra-pure aminosilane, set the temperature at 55°C, and the time is 35min. way to aminate the substrate.

[0037] The preparation process of substrates for biochips with high aldehyde functional group density: place amino sheets in a vacuum oven, vacuumize, open the vent hole, add ultra-pure adipaldehyde, set the temperature at 75°C, and use vacuum evaporation for 60 minutes. Aldehyde modified the substrate to obtain the aldehyde substrate.

[0038] Relevant testing steps are the same as in Example 1, and the performance testi...

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Abstract

The invention discloses a substrate for a high formyl group functional group density biological chip and a preparation method of the substrate. The method comprises the following steps: performing a polishing process by adopting ultra-white glass of low fluorescence background, so that the surface smoothness is improved; and realizing surface formylation by adopting a chemical grafting method, so that the adhesion is improved. The experiment proves that the substrate for the biological chip is small in fluorescence background and uniform in surface property, and the shape of sample points is always consistent in the long-term sample application process. The substrate is suitable for DNA, proteins and other types of probes and is suitable for point preparation of high-throughput chips, and the problem that a common substrate is non-uniform in sample application and low in adhesion is solved.

Description

[0001] technical field [0002] The invention relates to a substrate for biochips with high aldehyde functional group density and a preparation method thereof. Background technique [0003] As an important means of obtaining relevant information efficiently and on a large scale, biochip technology has been widely used in genomics research, single nucleotide polymorphism analysis, and clinical medical testing. [0004] Biochips generally choose silicon wafers, glass wafers, metal wafers, plastic wafers, etc. that have been treated accordingly as carriers. Glass slides have been widely used in the fabrication of DNA and protein chip microarrays due to their advantages of low cost, low fluorescence background and stable performance. In order to immobilize the probe on the surface of the glass slide, the surface of the glass slide must be physically and chemically modified. At present, there are many methods for chemically modifying the surface of glass slides, including amina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C03C17/30
Inventor 朱鹏
Owner 江苏贝思安特生物工程有限公司
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