Cortex albiziae saponin adjuvant and preparation method thereof, as well as vaccine preparation containing adjuvant and application thereof

A technology for saponin of Albizia Julibrissin and Albizia Julibrissin bark, which is applied in the application field of preparing vaccine preparations, can solve problems such as adverse reactions and poisoning, complex chemical structure, formation of granulomas, etc., and achieves easy quality control, simple preparation process, and low hemolysis. Effect

A technology for saponin of Albizia Julibrissin and Albizia Julibrissin bark, which is applied in the application field of preparing vaccine preparations, can solve problems such as adverse reactions and poisoning, complex chemical structure, formation of granulomas, etc., and achieves easy quality control, simple preparation process, and low hemolysis. Effect

CN103768593AInactive Publication Date: 2014-05-07ZHEJIANG UNIV
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  • Cortex albiziae saponin adjuvant and preparation method thereof, as well as vaccine preparation containing adjuvant and application thereof
  • Cortex albiziae saponin adjuvant and preparation method thereof, as well as vaccine preparation containing adjuvant and application thereof
  • Cortex albiziae saponin adjuvant and preparation method thereof, as well as vaccine preparation containing adjuvant and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1: Preparation of Albizia Julibrissin

[0045] Preparation of Total Saponins from Albizia Julibis

[0046] Medicinal platycodon grandiflorum powder 1 kg, reflux extraction with 70% ethanol for 3 times, each time for 2 hours, filter, combine filtrate, recover ethanol under reduced pressure, add appropriate amount of water to suspend the extract, extract with ether until the ether liquid is nearly colorless . The aqueous layer was extracted with water-saturated n-butanol until the extract was nearly colorless. The extracts were combined, recovered under reduced pressure, and concentrated to obtain the alcohol extract. The alcohol extract was passed through a D101 macroporous adsorption resin column, and then eluted with water and 75% ethanol in sequence, and the eluate was checked by thin-layer chromatography. The fractions with the same spots were combined, recovered under reduced pressure, concentrated, and freeze-dried to obtain acacia bark saponins. Crud...

Embodiment 2

[0053] Example 2 Hepatitis B vaccine preparation containing saponin adjuvant

[0054] Weigh 500 μg of the total saponins of Albizia juniperi, the active active fraction A of Albizia bark saponin adjuvant, or the active effective fraction B of Albizia juniperi saponin adjuvant, and 100 μg of recombinant hepatitis B virus surface antigen (rHBsAg), dissolve in 10 ml of normal saline, and dissolve in 0.22 μm Filtered through a microporous membrane, sterile aliquoted, 1 ml each. Each ml contains saponin 50μg and rHBsAg 10μg.

Embodiment 3

[0055] Example 3 Hepatitis B vaccine preparation containing aluminum gel and saponin adjuvant

[0056] Weigh an appropriate amount of total saponins of Albizia juniperi, the active active part A of Albizia bark saponin adjuvant, or the active effective part B of albizia juniperi saponin adjuvant, dissolve it in normal saline to make a solution with a concentration of 100 μg / ml, and filter it through a 0.22 μm microporous membrane filter. The saponin solution is mixed with an equal volume of aluminum-containing adjuvanted hepatitis B surface antigen vaccine stock solution (each ml contains 20 μg of HBsAg and 500 μg of aluminum hydroxide), mixed evenly, and aseptically dispensed, 1 ml per tube. Each ml contains saponin 50μg, HBsAg 10μg and aluminum hydroxide 250μg.

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Abstract

The invention relates to a cortex albiziae saponin adjuvant with the effect of an immunologic adjuvant and a preparation method thereof, as well as a vaccine preparation containing the saponin adjuvant, and the application of the adjuvant and the vaccine preparation in preparing vaccine preparations. The saponin comprises cortex albiziae total saponins extracted and separated from the traditional Chinese medicine cortex albiziae, a cortex albiziae saponin adjuvant active effective part A and a cortex albiziae saponin adjuvant active effective part B, can induce local tissues to generate cytokine and chemokine, can recruit immune cells to an injection part, can induce a body to simultaneously generate Th1 and Th2 type immune responses, is capable of inducing a body to generate stronger cell immune and body fluid immune responses on the vaccine than an existing alumina gel in the prior art, can be used as an immune adjuvant of a plurality of vaccines to play a perfect immune effect. The vaccine by using the saponin as an adjuvant is simple in preparation process and method, easy in quality control and convenient to use, can be can be frozen for storage.

Description

technical field [0001] The present invention relates to the total saponins of Albizia Julibrissin with the function of immune adjuvant extracted and separated from the traditional Chinese medicine Albizia Julibrissin, the active effective part A of the adjuvant of Albizia Julibis saponin and the active effective part B of the adjuvant of Albizia Julibis saponin, and its preparation method and containing the saponin Adjuvanted vaccine formulations, and their use in the preparation of vaccine formulations. Background technique [0002] Vaccination is the most economical and effective means to prevent and control infectious diseases. New vaccines such as DNA vaccines, recombinant vaccines, and synthetic peptide vaccines are a type of biological preparations that have developed rapidly in recent years. Compared with conventional vaccines, they have the advantages of high antigen purity, strong specificity, and good safety. However, these novel vaccines have weak immunogenicity ...

Claims

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Application Information

Patent Timeline
07 May 2014
Publication
CN103768593A
IPC
A61K39/39; A61K36/48; A61P37/04
Inventors
孙红祥