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Tissue engineered bone constructed from allogeneic bone marrow mesenchymal stem cells and application thereof

A bone marrow mesenchymal and tissue engineered bone technology, applied in prosthesis, medical science, etc., can solve the problem of limiting the clinical application of tissue engineered bone, affecting the proliferation ability and multidirectional differentiation potential of bone marrow mesenchymal stem cells, and delaying the best of diseases Treatment time and other issues, to achieve good nutrition supply and metabolic environment, improve the success rate of preparation and product quality, and the effect of good proliferation and differentiation ability

Inactive Publication Date: 2014-05-07
PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
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Problems solved by technology

At present, autologous bone marrow mesenchymal stem cells (BMSCs) are ideal seed cells in the process of tissue engineering bone construction, but the number of bone marrow mesenchymal stem cells in autologous bone marrow gradually decreases with age; clinically, most patients with bone defects Different disease states, as well as the application of treatment measures such as radiation and drugs, may affect the proliferation ability and multi-lineage differentiation potential of bone marrow mesenchymal stem cells to varying degrees; Construction and other processes may also delay the optimal treatment time of the disease, thus limiting the clinical application of tissue engineered bone

Method used

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  • Tissue engineered bone constructed from allogeneic bone marrow mesenchymal stem cells and application thereof
  • Tissue engineered bone constructed from allogeneic bone marrow mesenchymal stem cells and application thereof
  • Tissue engineered bone constructed from allogeneic bone marrow mesenchymal stem cells and application thereof

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Embodiment

[0024] 1. Isolation and culture of beagle bone marrow mesenchymal stem cells

[0025] Beagle dogs, 12 months old, were anesthetized by intramuscular injection of ketamine / Sumianxin (the two were mixed at a volume ratio of 2:1) at a dose of 0.12ml / kg. Routine skin preparation, disinfection, and draping. A heparinized 16-gauge bone needle was used to penetrate the bone marrow cavity at the posterior superior iliac crest, and then a 20-ml syringe containing 1 ml of heparin (50 U / ml) was used to draw the bone marrow fluid under negative pressure4 -ml, add 4ml Hank's solution and mix well, then carefully add to the 15-ml centrifuge tube containing 4-ml Ficoll lymphocyte separation solution along the tube wall, and centrifuge at 2000-r / min for 20 minutes. Aspirate the white cloudy nucleated cell layer in the middle, resuspend in low-sugar DMEM medium, centrifuge at 1000-r / min for 5 minutes, discard the supernatant, repeat 2 times. After centrifugation, resuspend the cells with low-...

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Abstract

The invention discloses an allogeneic tissue engineered bone implant, which comprises a biodegradable material and bone marrow mesenchymal stem cells, wherein the bone marrow mesenchymal stem cells are in vitro cultured allogeneic bone marrow mesenchymal stem cells. The tissue engineered bone constructed from allogeneic bone marrow mesenchymal stem cells can repair bone defects, and the effect has no significant difference compared with conventional tissue engineered bone constructed from autologous bone marrow mesenchymal stem cells. The invention also provides a preparation method and application of the tissue engineered bone.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a tissue engineered bone constructed by allogeneic bone marrow mesenchymal stem cells and an application thereof. Background technique [0002] Bone defects caused by trauma, infection, tumor, congenital deformity and other reasons are common clinically. The current treatment methods mainly include autologous bone transplantation, allogeneic bone transplantation, artificial bone substitute filling, etc., but there are some shortcomings, such as autologous Bone transplantation has problems such as damage to the donor area, limited source and quantity; the bone healing process of allogeneic bone transplantation is slow and there are various degrees of rejection, and there is also a high possibility of infectious disease transmission; artificial bone substitutes Poor ability to repair bone defects due to lack of osteoinductive ability. However, tissue engineering bone technology h...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/38
Inventor 曹谊林肖苒王黔武京国
Owner PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
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