Method for improving anaerobic methanogenesis efficiency by using nano magnetite

An anaerobic methane production and nano-magnet technology, which is applied in waste fuel, fermentation and other directions, can solve the problems of increasing the operating cost of biogas projects, long biogas fermentation period, limiting the rate of biogas fermentation process, etc., so as to improve energy utilization efficiency and improve methane effect of concentration

Inactive Publication Date: 2014-05-07
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

However, due to the H in solution 2 Or the limitation of the diffusion rate of formic acid and the influence of agitation during the fermentation process, the spatial distance of the mutualistic flora largely limits the efficient and rapid tra

Method used

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  • Method for improving anaerobic methanogenesis efficiency by using nano magnetite
  • Method for improving anaerobic methanogenesis efficiency by using nano magnetite
  • Method for improving anaerobic methanogenesis efficiency by using nano magnetite

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Synthesize 10nm magnetite colloidal solution by co-precipitation method according to the prior art, and set it aside. Anaerobic digested sludge was selected as the inoculum, and the electron donor was a mixture of propionic acid and butyric acid (mass ratio, 1:1).

[0022] Add the above-mentioned magnetite with a final concentration of 2g / L, anaerobic digested sludge with a final concentration of 15g / L, propionic acid and butyric acid (5g / L each), and 150mL of medium In the anaerobic reactor with residual oxygen in the bottle, it is anaerobically fermented under the sealed conditions of pH=7.0 and 37°C.

[0023] At the same time, no magnetite was added as a control group (see figure 1 ).

[0024] Depend on figure 1 It was shown that the addition of magnetite led to a 90% increase in methane production and a 1.9-fold increase in methane production rate compared with the group without magnetite addition, and the enriched mutualistic microbial flora had significant elec...

Embodiment 2

[0029] Synthesize 10nm magnetite colloidal solution by co-precipitation method according to the prior art, and set it aside. Paddy soil was selected as the inoculum, and the electron donor was propionic acid.

[0030] Add the above-mentioned magnetite with a final concentration of 2g / L, paddy soil with a final concentration of 10g / L, propionic acid (5g / L) and medium (30mL) into the anaerobic bottle purged with high-purity nitrogen to remove residual oxygen In the anaerobic reactor, the anaerobic fermentation was carried out under the sealed conditions of pH=7.0 and 37°C.

[0031] At the same time, no magnetite was added as a control group (see image 3 ).

[0032] Depend on image 3 It was shown that the addition of magnetite led to a 7-fold increase in methane production and a 12-fold increase in the methane generation rate compared to the group without magnetite addition.

[0033] Properties of paddy soil: pH6.3, organic matter 20.1g / kg.

Embodiment 4

[0035] Synthesize a 30nm magnetite colloidal solution by co-precipitation method according to the prior art, and set it aside. Paddy soil was selected as the inoculum, and the electron donor was butyric acid.

[0036] Add the above-mentioned substances into the culture medium, and then transfer to the anaerobic reactor that purged with high-purity nitrogen to remove the residual oxygen in the anaerobic bottle. Add 8g of butyric acid and 3g of magnetic Iron Ore and 20g of Paddy Soil. Anaerobic fermentation was carried out in a reactor under sealed conditions of pH=7.0 and 37°C.

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Abstract

The invention belongs to the field of biomass energy sources and relates to a method for improving anaerobic methanogenesis efficiency by using nano magnetite. The method takes a short-chain fatty acid as an electron donor and the nano magnetite is used as an electron carrier; under an anaerobic fermentation cultivation condition, the short-chain fatty acid is degraded so as to improve syntrophic oxidization methanogenesis efficiency of the system. With the adoption of the method, the energy source utilization efficiency of the short-chain fatty acid can be obviously improved. The method has important meanings on the improvement of the operation efficiency of a methanogenesis reactor and also has good economic benefits.

Description

technical field [0001] The invention belongs to the field of biomass energy, and relates to a method for improving the efficiency of anaerobic methane production by using nano-magnetite. Background technique [0002] Mutualism is a closely linked mutualistic cooperative behavior among different microbial species, and it is an essential part of the methanogenic ecosystem. Mutualism plays a very important role in the anaerobic fermentation methanogenesis system. The conversion of short-chain fatty acids (such as propionic acid and butyric acid) into methane requires the indirect force between mutualistic bacteria and methanogens, which is through interspecific electron transfer. to realise. [0003] In the anaerobic methanogenesis system, the interoperability relationship mainly exists between interoperating bacteria and methanogens. It is generally believed that during the methanogenic process, short-chain fatty acids (such as propionic acid and butyric acid) produced by ac...

Claims

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Application Information

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IPC IPC(8): C12P5/02
CPCY02E50/30
Inventor 郭荣波杨智满王传水许晓辉戴萌
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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