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Method for identifying chrysanthemum heat resistance related differential protein

A technology of differential protein and chrysanthemum, applied in the field of proteomics, can solve the problems of time-consuming and labor-intensive, increase the difficulty of experiments, etc., and achieve the effect of improving heat resistance

Active Publication Date: 2014-05-07
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

[0003] However, it is not only time-consuming and labor-intensive to obtain heat-resistant related proteins through genes such as differential genes, but also greatly increases the difficulty of experiments due to many changes from the gene level to the protein level.

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  • Method for identifying chrysanthemum heat resistance related differential protein
  • Method for identifying chrysanthemum heat resistance related differential protein
  • Method for identifying chrysanthemum heat resistance related differential protein

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Embodiment

[0019] Example Identification method for chrysanthemum heat resistance-related differential proteins

[0020] Include the following steps:

[0021] 1. Select the heat-resistant chrysanthemum chrysanthemum variety chrysanthemum brain and the heat-sensitive chrysanthemum variety "late powder" that are free from diseases and insect pests and grow vigorously, and cut the foot buds of the same size for cutting propagation. The cuttings are planted in peat: perlite = 1:1 (weight ratio) cultured in the matrix for 3 months. Then the healthy plants without pests and diseases were moved to the artificial climate incubator, and cultivated for 24 hours under the conditions of daytime and night temperature of 20°C / 18°C. Then select some of them and place them under the conditions of 40°C during the day and a relative humidity of 75% for 6 hours of heat stress, and the control condition is 20°C during the day and a relative humidity of 75%. Chrysanthemum leaves were randomly selected and ...

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Abstract

The invention provides a method for identifying chrysanthemum heat resistance related differential protein. The method comprises the following steps: respectively performing heat shock treatment on a heat-resisting chrysanthemum variety and a heat-labile chrysanthemum variety for 6 hours; extracting a treated leaf protein sample by using a TCA (Trichloroacetic Acid) / acetone precipitation method; rapidly identifying the differential protein by using an iTRAQ (Isobaric Tags For Relative And Absolute Quantitation) and mass spectrum series technology, and performing bioinformatics analysis. Aiming at the chrysanthemum, the invention provides a method for rapidly and accurately identifying the chrysanthemum heat resistance related differential protein, and the method provides research basis for improving the heat resistance of the heat-labile chrysanthemum, thus improvement of the chrysanthemum variety is realized.

Description

technical field [0001] The invention relates to the field of proteomics, in particular to a method for identifying heat-resistant related differential proteins of chrysanthemums. Background technique [0002] Chrysanthemum is one of China's top ten traditional famous flowers and one of the world's four major cut flowers. It has a wide variety, rich colors, changeable flower shapes, and various postures. It plays an extremely important role in the cultivation and application of flowers in the world. But its optimum growth temperature is 15~25℃, when the temperature exceeds 25℃, especially in summer, it is not conducive to the growth of chrysanthemums. High temperature can cause reproductive disorders, delayed flowering, base leaf defoliation, loose plant type, etc. Therefore, identifying related genes, understanding their expression patterns and functions has become a crucial means, and based on this, effective measures can be taken to improve plant heat tolerance. [0003]...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/89
Inventor 孙明张杨张启翔
Owner BEIJING FORESTRY UNIVERSITY
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