Method for purifying crude product of heparin sodium

A purification method and heparin sodium technology are applied in the fields of heparin sodium extraction and biological enzymolysis, which can solve the problems of high discharge concentration of waste liquid, pollution and low yield, and achieve the effect of removing impurities and simple process.

Active Publication Date: 2014-05-21
QINGDAO JIULONG BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This traditional process has the defects of low yield, unstable harvest, ...

Method used

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Examples

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Embodiment 1

[0014] (1) Dissociation (enzymolysis): Weigh 1kg of small intestinal mucosal fluid of healthy pigs (solid-liquid ratio is about 1:9) and pump it into the reaction tank, adjust the pH to 9.5 with industrial alkali, and add 40g of sodium chloride , add 5g of active enzyme, mix well, raise the temperature to 48°C, stir at constant temperature, dissociate for 2 hours, keep the pH constant during the dissociation process, raise the temperature to 85°C, keep warm for 12 minutes, and then cool immediately.

[0015] (2) Filtration: The cooling liquid was filtered twice with a suction filter, successively using 2 μm and 0.22 μm microporous membranes to remove impurities to obtain the filtrate.

[0016] (3) Enzymolysis of protein: adjust the pH of the filtrate to 8.0, then raise the temperature to 48°C, add 5g of active enzyme, keep the temperature at 48°C and stir for 6 hours, control the pH value of the reaction system during the enzymolysis process, and then rapidly The temperature w...

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Abstract

The invention discloses a method for purifying a crude product of heparin sodium. The method adopts the small intestinal mucosa of a healthy pig as a main raw material and obtains a crude product of heparin sodium by the steps of dissociation (enzymolysis), filtration, protein enzymolysis, protein removal and precipitation, dehydration and drying. The method has the advantages of short production cycle, low production cost and high yield, does not generate toxic substances, and is good for environmental protection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting heparin sodium, which is a method for purifying heparin sodium by using pig small intestinal mucosa as a raw material through biological enzymatic hydrolysis technology. Background technique [0002] Heparin sodium is a mucopolysaccharide sulfate anticoagulant drug, which is the sodium salt of aminodextran sulfate extracted from the intestinal mucosa of pigs or cattle. The first drug for thromboembolic diseases such as deep venous thrombosis. With the deepening of research, it has been found that heparin sodium not only has anticoagulant, antithrombotic and blood lipid adjustment functions, but also has anti-inflammatory, anti-allergic, anti-viral, and anti-inflammatory effects. Cancer and other biological functions. [0003] At present, the extraction of crude heparin sodium generally involves scraping the small intestine of animals. The mucus obtained after...

Claims

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Application Information

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IPC IPC(8): C08B37/10
Inventor 刘乃山陆玉梅迟培升夏衬来
Owner QINGDAO JIULONG BIO PHARMA
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