Hericium erinaceus large-molecular polysaccharide and preparation method thereof

A high molecular weight, Hericium erinaceus technology, applied in the field of Hericium erinaceus high molecular weight polysaccharide and its preparation, can solve the problems of less research on the large molecular weight part, unfavorable large-scale production and application, complicated operation process, etc., to enhance the immunity of the body. , low cost and simple preparation method

Active Publication Date: 2014-05-28
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the research on Hericium erinaceus polysaccharides, different scholars, different species, different extraction, separation and purification methods have different results, but the current research on Hericium erinaceus polysaccharides is mainly concentrated on the molecular weight 1-10KDa part, and the research on the large molecular weight part less, and the separation and purification methods generally adopt methods such as water extraction and alcohol precipitation, ion exchange, resin decolorization, gel column purification and other methods. Large-scale production application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Water extraction and alcohol precipitation: take the fresh fruiting body of Hericium erinaceus as raw material, cut it into pieces of about 2cm, weigh 2kg of raw material and extract it with boiling water, filter the extract with a 200-mesh filter, and collect it by centrifugation at 4000rpm for 30min Clear, concentrated under reduced pressure until the soluble solid content is 18°Brix (the soluble solid content is measured by a hand-held digital display sugar meter.), after cooling to room temperature, add absolute ethanol until the ethanol mass percentage content is 70%, 4 ℃ Let stand overnight, centrifuge at 10000g for 15min to collect the precipitate.

[0031] 2. Freezing: dissolve the above precipitate with water, centrifuge at 10,000g for 15min to remove impurities, and freeze the supernatant at -20°C. 3. Dissolving: put the above frozen solution in a water bath at 80°C to heat and thaw, centrifuge at 10000g for 20min to collect the precipitate;

[0032] 4. Et...

Embodiment 2

[0038] 1. Water extraction and alcohol precipitation: take the fresh fruiting body of Hericium erinaceus as the raw material, cut it into pieces of about 2cm, weigh 1kg of the raw material and extract it with boiling water, filter the extract with a 200-mesh filter, and collect it by centrifugation at 4000rpm for 30min Clear, concentrated under reduced pressure to a soluble solid content of 18°Brix, cooled to room temperature, added absolute ethanol until the ethanol mass percentage concentration was 60%, stood overnight at 4°C, centrifuged at 10,000g for 15min to collect the precipitate.

[0039] 2. Freezing: dissolve the above precipitate with water, centrifuge at 10,000g for 15min to remove impurities, and freeze the supernatant at -20°C.

[0040] 3. Dissolving: put the above frozen liquid in a 100°C water bath to heat and thaw, centrifuge at 10000g for 20min to collect the precipitate;

[0041] 4. Ethanol precipitation and washing: dissolve and dilute the above precipitate...

Embodiment 3

[0047] Determination of the activity of stimulating macrophages to release NO in vitro

[0048] The RAW264.7 bone marrow macrophage cell strain in this example was purchased from the American National Culture Collection (ATCC number TIB-71 TM );

[0049] In this example, DMEM and RPMI1640 were purchased from GIBCO Company.

[0050] Preparation of test samples

[0051] Accurately weigh the Hericium erinaceus polysaccharide samples prepared in Examples 1 and 2 in a sterilized eppendorf tube, and use PBS buffer solution to prepare a concentration of 5 mg / mL. After fully dissolving, centrifuge at 12,000 rpm / min for 30 min, transfer to a new sterile eppendorf tube under aseptic conditions, and dilute the sample to a final concentration of 50, 200, and 500 μg / mL for use. The negative control was PBS buffer, and the positive control was 10 μg / mL LPS solution.

[0052] Preparation of mouse RAW264.7 macrophages

[0053] with DMEM medium at 37°C, 5% CO 2 After subculture under condi...

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Abstract

The invention provides a hericium erinaceus large-molecular polysaccharide and a preparation method thereof. The hericium erinaceus large-molecular polysaccharide is prepared by virtue of water extraction, alcohol precipitation, refrigeration and dissolution. The hericium erinaceus large-molecular polysaccharide provided by the invention has the molecular weight more than 1 million, is few in pigment and has polysaccharide content which is greatly increased by more than 80%; the hericium erinaceus large-molecular polysaccharide provided by the invention plays an obviously promotion role on an RAW264.7 macrophage cell line in releasing NO, thereby enhancing the immunity and anti-tumor capacity of an organism.

Description

technical field [0001] The invention relates to the field of extraction and purification of edible and medicinal fungi, in particular to a large molecular weight polysaccharide from Hericium erinaceus and a preparation method thereof. Background technique [0002] Hericium erinaceus (Hericium erinaceus) is a kind of edible and medicinal fungus, with tender and delicious meat and rich nutrition. In ancient times, people listed it as one of the "Four Famous Dishes" together with soft-shelled turtle fish, sea cucumber, and bear's paw thorn, and it has the reputation of "mountain delicacy Hericium, seafood bird's nest". It has been reported in the literature that Hericium erinaceus has various pharmacological effects such as anti-ulcer, anti-inflammation, anti-tumor, hypoglycemic, anti-aging, anti-mutation, and liver protection. Hericium erinaceus polysaccharides, as one of the most important active ingredients in Hericium erinaceus, have various biological activities and pharm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 吴迪杨焱唐川刘艳芳张劲松汪雯翰颜梦秋唐庆九唐传红冯娜周帅贾薇张忠冯杰
Owner SHANGHAI ACAD OF AGRI SCI
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