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Preparation method for fluorescent nano-cluster based on rare-earth metal cerium and application of fluorescent nano-cluster

A fluorescent nanocluster, rare earth metal technology, applied in the fields of nanotechnology, nanotechnology, nanotechnology for materials and surface science, can solve the problems of expensive sensitivity, not high enough, and complicated process for ordinary patients, and achieve good biological phase. Capacitance, broad application prospects, and the effect of high fluorescence properties

Active Publication Date: 2014-05-28
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are many methods for tumor imaging, such as nuclear magnetic resonance, PET-CT, radioisotopes, etc., but the process is complicated and too expensive for ordinary patients and the sensitivity is not high enough

Method used

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  • Preparation method for fluorescent nano-cluster based on rare-earth metal cerium and application of fluorescent nano-cluster
  • Preparation method for fluorescent nano-cluster based on rare-earth metal cerium and application of fluorescent nano-cluster
  • Preparation method for fluorescent nano-cluster based on rare-earth metal cerium and application of fluorescent nano-cluster

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Experimental program
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Effect test

Embodiment 1

[0026] The first step is to prepare a certain concentration of reduced glutathione aqueous solution, gold reagent and cerium reagent aqueous solution;

[0027] In the second step, mix 1.5 to 10 mmol / L reduced glutathione aqueous solution and 0.5 to 5 mmol / L gold reagent evenly, and shake fully until the color of the solution becomes colorless;

[0028] The third step is to put the mixed solution in a water bath and heat it for a period of time, then add 0.025 to 5 mmol / L cerium reagent to the gold reagent mixed solution, continue to react for a period of time, then take it out from the water bath, cool to obtain fluorescence Excellent gold-cerium nanocomposite material; the heating time is 0.5 to 4 hours, the heating temperature is 37 to 90°C, and the ratio of glutathione to gold reagent and cerium reagent can be adjusted to obtain nanomaterials with different fluorescence yields.

Embodiment 2

[0030] 1) Prepare a certain concentration of gold reagent and cerium reagent aqueous solution;

[0031] 2) Mix 0.0001 to 1 mmol / L cerium reagent with 0.0001 to 1 mmol / L gold reagent evenly;

[0032] 3) Liver cancer cells (HepG2) were selected as the research object, and the experimental group divided the liver cancer cells (HepG2) in the logarithmic growth phase at a ratio of 1.6×10 5 The density of cells / well was inoculated in 6-well plates, and after 8 hours of culture, sterilized and diluted with fresh sterile DMEM medium was added containing gold-cerium mixed solution with a concentration of 0.0001 to 1 mmol / L and logarithmic HepG2 cells in the growth phase were co-incubated for 4-48 hours (37 °C, 5 % CO2, RH 95%); in the control group, the liver cancer cells (HepG2) in the culture 5 Cells / well were seeded in 6-well plates and cultured under the same conditions as the experimental group. Add phosphate buffer solution (PBS, pH=7.2) to each well of the experimental group a...

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Abstract

The invention discloses a preparation method for fluorescent nano-cluster based on rare-earth metal cerium and application of the fluorescent nano-cluster, and relates to a preparation method for synthesizing cerium gold nano-cluster greenly based on rare-earth metal element cerium and application of the cerium gold nano-cluster in imaging and detecting of tumour. Related nano-cluster materials have the characteristics of small size, high stability and the like, have favorable biocompatibility and stable fluorescence characteristic in practical application, and are free from congregation when the nano-cluster materials are placed in an ambient temperature for a long time, and stable in fluorescent property; the method is simple, convenient, and easy to operate; the synthesized nanometer material is low in toxicity, easy to separate and refine, high in signal to noise ratio in process of detecting and imaging of related biology, and high in sensitivity and specificity, and has spacious medical clinical application value and prospect.

Description

technical field [0001] The invention discloses a preparation method and application of fluorescent nano-clusters based on rare earth metal cerium. It specifically involves using reduced glutathione as a reducing agent, the preparation method of cerium and gold cerium nanoclusters based on the rare earth metal element cerium green synthesis and its application in tumor imaging and detection, and the application of related fluorescent nanoprobes to tumors Targeted imaging and detection of heavy metal ions in the environment; secondly, using the physiological environment of tumors, fluorescent gold-cerium nanoclusters were synthesized in situ to realize real-time dynamic imaging analysis of living tumor cells. Background technique [0002] In terms of environmental pollution, heavy metals refer to heavy metals with significant biological toxicity such as mercury, cadmium, lead, and "metalloids"-arsenic. Among them, lead, mercury, arsenic, etc. are extremely poisonous to the hu...

Claims

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Application Information

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IPC IPC(8): C09K11/77A61K49/00G01N21/64B82Y30/00B82Y40/00
Inventor 王雪梅革伟张园园
Owner SOUTHEAST UNIV
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