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A kind of method that improves Penicillium to produce cellulase

A technology of cellulase and Penicillium production, which is applied in the field of improving the production of cellulase from Penicillium, can solve the problems of cumbersome steps and high cost, and achieve the effect of improving enzyme activity and simple method

Inactive Publication Date: 2016-02-03
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main methods to improve the enzyme activity of cellulase-producing bacteria are mutation breeding and the use of genetic engineering to construct high-yielding cellulose-degrading bacteria. These methods are cumbersome and costly.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of buckwheat extract

[0022] Weigh 20g of buckwheat, add 200ml of distilled water and soak for 1 hour, heat it to boiling in an electric furnace, adjust the heating temperature, and maintain a slightly boiling state for 30 minutes. At this time, the buckwheat has swelled and turned white. The initial buckwheat extract is cooled to room temperature and then ultrasonically broken. The ultrasonic breaker is set to power 50%, total time 30min, ultrasonic time 5s, and intermittent time 3s. Finally, centrifuge at 7000r / min for 15min, and take the supernatant to obtain the buckwheat extract.

Embodiment 2 1

[0023] Example 2 Primary seed culture

[0024] Accurately weigh 0.5g peptone, 1.0g yeast extract, 0.05g magnesium sulfate, 0.2g potassium dihydrogen phosphate, 0.4g potassium dihydrogen phosphate in a 250ml Erlenmeyer flask and dissolve it with 80ml distilled water, weigh 1.0g glucose, and dissolve it with 20ml distilled water After the two are sterilized and cooled, in the ultra-clean workbench, pour the glucose solution into a 250ml Erlenmeyer flask, and inoculate a loop of Penicillium spores on the PDA medium, and incubate at 28°C and 190r / min for 48h to obtain Primary seed culture solution.

Embodiment 3

[0025] Example 3 Fermentation culture

[0026] Accurately weigh 0.2g peptone, 0.025g yeast extract, 0.1g ammonium sulfate, 0.2g potassium dihydrogen phosphate, 1.5g starch, 0.015g magnesium sulfate, 0.015g calcium chloride, 50ml distilled water, 1.8g microcrystalline cellulose, 5ml of buckwheat extract, as for the 250ml Erlenmeyer flask, repeat the same for three times. After sterilization and cooling, in a sterile environment, inoculate 5 ml of the first-grade seed solution prepared in Example 2, and in a shaker, culture at 28° C. and 185 r / min for 6 days. After collecting the crude enzyme solution, the CMC enzyme activity was determined to be 156.65 U / ml, and the filter paper enzyme activity was 24.78 U / ml.

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PUM

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Abstract

The invention discloses a method for improving enzyme activity of cellulase produced from penicillium. The method comprises the following steps: inoculating rejuvenated penicillium spores in a PDA medium into a penicillium medium for culture so as to obtain a primary seed culture solution; and inoculating the primary seed culture solution into a fermentation medium with a buckwheat extract for culture. According to the invention, the buckwheat extract is used as an additive for the penicillium fermentation medium, so the enzyme activity of cellulase produced from penicillium is greatly improved; the method has the advantages of easiness, practicability, low cost and good effects.

Description

Technical field [0001] The invention relates to a method for improving enzyme activity, in particular to a method for improving cellulase production by Penicillium. Background technique [0002] With the depletion of traditional energy reserves, the energy crisis has become an important factor restricting the rapid development of countries, and the search for and vigorously develop sustainable renewable resources has become one of the global hot spots. Cellulose is the largest renewable resource in the world, mainly in the cell walls of plants. The annual global photosynthesis produces plant biomass as high as 1.145×10 12 Tons, and cellulose resources account for 60% to 80% of the earth’s total biomass. The use of cellulose to produce new clean energy and other biochemical by-products is a hot spot of research and development today. [0003] my country has abundant cellulose resources. The straw husk alone can reach more than 700 million tons per year. Among them, corn straw (35%)...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12R1/80
CPCC12N9/2437C12N9/2445C12Y302/01004C12Y302/01021C12Y302/01091
Inventor 曾柏全冯金儒
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY