A kit and method for detecting human parvovirus b19

A human parvovirus and parvovirus technology, applied in the biological field, can solve the problem of high detection cost, and achieve the effects of good repeatability, good application prospects and high accuracy
CN103820581BActive Publication Date: 2016-01-13CHENGDU RONGSHENG PHARMA

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHENGDU RONGSHENG PHARMA
Publication Date
2016-01-13

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Abstract

A kit for detecting human Parvoviridae B19, the kit comprising a primer pair shown by nucleotide sequence such as SEQ ID NO: 1-2, and amplifying the genes from human Parvoviridae B19. The kit can amplify gene fragments of human Parvoviridae B19 and has strong specificity, high sensitivity, good repeatability, and is used for quick testing of blood products and has good application prospects.
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Description

technical field

[0001] The invention relates to a kit for detecting human parvovirus B19, which belongs to the field of biotechnology. Background technique

[0002] Human parvovirus B19 (Humanparvovirus B19) is a non-enveloped single-stranded DNA virus with a diameter of about 18-26nm. fetal death. Generally spread through the respiratory tract, but also through blood transfusion, blood products and blood-fetal route. Human parvovirus B19 has a small diameter and no envelope, so it is very difficult to inactivate and remove it. Therefore, it is necessary to establish a B19 detection method to effectively detect whether blood products contain B19, so as to improve the safety of blood products.

[0003] At present, B19 detection methods are divided into three major technologies: serology, molecular biology and histology. Among them, serological tests represented by ELISA and molecular biological tests represented by PCR are relatively easy. However, because the B19 virus i...

Claims

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