Preparation method of concentrated culture solution of nitzschia closterium minutissima allen et nelson and plastic barrel cultural method
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A culture solution and crescent technology, which is applied in the field of plastic barrel culture and the preparation of concentrated culture solution of small crescent rhomboid diatoms, can solve the problems of unsuitable expansion stage, large one-time investment, low economic benefit, etc. The effect of growth, low economic benefit and large investment
Active Publication Date: 2014-06-04
山东中科绿碳科技有限公司
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Regarding the culture method of small crescent rhomboid diatoms, the traditional open cement pond culture has a large scale of cultivation and a high total output, but the disadvantages are also very obvious: easy to pollute, large area, greatly affected by the weather, difficult to control, one-time investment Large, low per unit yield, slow effect, low economic benefit, not suitable for the expansion stage
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[0015] 1) Take NH 4 NO 3 5 g, CaCO 3 1.4 g, K 2 HPO 4 4 g, Na 2 SiO 3 9H 2 O0.5 g, MgSO 4 ·7H 2 O25g, NaHCO 3 4 grams, 100 ml of f / 2 trace element solution, 100 ml of human urine, 150 ml of sea mud extraction solution, 10 ml of cow dung leaching solution, and 15 ml of sheep manure leaching solution were added in sequence to a Erlenmeyer flask with 1000 ml of seawater, and boiled for 10 Minutes, cool down; then add 400 IU of 920 plant growth stimulant, 100 ml of f / 2 vitamin solution, shake well, seal with plastic wrap, and store in 4 refrigerators for later use. Note that 920 plant growth stimulant and f / 2 vitamin solution cannot be boiled and sterilized together, because the two are biologically active substances, which are easy to decompose and denature when exposed to high temperature;
[0016] 2) Because the concentration of the concentrated culture solution is very high, use it after diluting it according...
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Abstract
The invention discloses a preparation method of a concentrated culture solution of nitzschia closterium minutissima allen et nelson and a plastic barrel cultural mode. The conventional preparation method of the culture solution is immediate preparation; the culture solution is prepared once for use at one time according to the use level, so that the conventional method is time-, work- and labor-consuming, troublesome to weigh and great in error. According to the invention, the concentrated liquor is adopted by the inventor for use for many times, so that the culture solution is simple to operate, convenient to store and convey, suitable for scaled, industrialized, commercialized and professional production, and very convenient. The inventor optimizes the conventional formula and supplements NaHCO3, 920 plant growth stimulin, human urine, sea mud extracting liquor, cow dung and sheep manure lixivia and the like, so that the culture solution is more complete, balanced and abundant in nutrition. As for the cultural mode, the inventor adopts a household mineral water barrel cultural mode to replace conventional open cement pit culture, so that the cultural method is easy to operate, difficult to be polluted, easy to master, agile and diverse, difficult to scratch, safe and reliable, good in transmission of light, low in cost and fast in growth, and the output is improved by 150%.
Description
technical field [0001] The invention belongs to the field of marine microalgae cultivation, and in particular relates to a method for preparing a concentrated culture solution of small crescent rhomboid diatoms and a method for cultivating them in a plastic bucket. Background technique [0002] Small Crescent Nitzophrenia belongs to Diatomphyta, Plumenaea, Dirhomboides, Nitzphyceae, Nitzphylla, the center of the cell expands into a spindle shape, and the two ends are acuminate, like a crescent. The cells are 12-23 μm long and 2-3 μm wide, with a nucleus in the center. Chromosome yellowish brown, two pieces, located on both sides of nucleus. Distributed in seawater, brackish and freshwater waters of the world, it occurs in every sea area of my country. The optimum salinity range is 25-32%, the optimum temperature is 15-20°C, and the growth will stop if the water temperature exceeds 28°C; the optimum light intensity is 3000-8000lx, avoid direct sunlight during cultivation;...
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