Chemosynthetic extracellular region gene fragment of streptococcus pneumonia PspA protein, and expression and application thereof
A Streptococcus pneumoniae and gene fragment technology, applied in the field of genetic engineering technology, can solve problems such as large variability in the alpha helix region, and achieve the effects of avoiding interference and easy purification
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[0048] Detailed description of the embodiment of the present invention:
[0049] Epitope Analysis, Gene Synthesis and Expression of Surface Protein A Antigen of Streptococcus pneumoniae
[0050] Through the computer analysis of the entire amino acid sequence of the PspA protein of Streptococcus pneumoniae, the strong antigenic epitope in the PspA protein is screened out, and the codons preferred by the bacteria are selected to chemically synthesize a new gene fragment of the strong antigenic epitope of the PspA protein. Cloning the gene fragment into the plasmid pGEX4T-2 Bam HI / Eco The RI site, consistent with the translation frame of the start codon on the vector, can express a fusion protein. The recombinant plasmid was transformed into Escherichia coli BL21 (DE3), and the engineered bacteria that highly expressed PspA protein were obtained by screening, and the expressed PspA protein accounted for about 30% of the total bacterial protein.
[0051] Materials and Me...
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