Reagent for detecting blood calcium of milk in semi-quantitative way

Abstract

The invention discloses a reagent for detecting the blood calcium of milk in a semi-quantitative way. The reagent comprises a reagent 1, a reagent 2 and a reagent 3 which are independently packaged, wherein the reagent 1 comprises 10mu L of triethanolamine with the volume percent of 20%, and 2.5mu L of sodium sulfide solution with the mass percent of 2%; the reagent 2 comprises 10mu L of ammonia water-ammonia chloride mixed buffer solution with pH of 10 and 10mu L of sodium hydroxide with concentration of 2 mol / L; the reagent 3 comprises 150mu L of ethylenediamine tetraacetic acid disodium salt with concentration of 2.00-2.10 mol / L, 150mu L of Cal-Red with concentration of 2.00-2.10 mol / L and 30mu L of lemon yellow with concentration of 2.00-2.10 mol / L. The reagent does not need a special instrument and can be directly used; furthermore, the clinical difficulties of veterinarian can be overcome; the reagent can be operated without needing a special technicist, can be observed by naked eyes and can also be used by a common worker.

Description

technical field [0001] The invention relates to a reagent for semi-quantitatively detecting dairy cow blood calcium. Background technique [0002] Calcium ion is an important cation in the body. Calcium ion in the blood has important physiological activities. As a constant mineral element in the body, it not only participates in the coagulation of blood, the conduction of nerve impulses, the contraction of muscles, and the formation of dense capillary endothelium And maintain the normal permeability of cell membrane, but also the activator of many enzymes, can promote the secretion of insulin, catecholamine, adrenal corticosteroid and saliva. The normal blood calcium range is maintained by the interaction of a stable feedback system composed of vitamin D3, parathyroid hormone and calcitonin in the body. When the content of calcium ions fluctuates, the corresponding physiological functions will change, which will lead to many diseases. For example, when the serum calcium co...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a reagent for semi-quantitative detection of blood calcium in dairy cows in order to solve the problems of complex operation, high cost, and the need for specialized technical personnel to complete the current quantitative detection of blood calcium.