Method of preparing high F-value collagen peptide by hydrolyzing anglerfish fishskins

A collagen peptide and collagen protein technology, which is applied in the field of hydrolyzing anglerfish skin to prepare high F-value collagen peptides, can solve the problems of ineffective use of anglerfish skin waste, lack of preparation of high-F value fish collagen peptides, etc., and achieve effective Good for human body absorption, relatively small molecule, high value effect

Active Publication Date: 2014-06-11
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem that the existing waste of anglerfish skin has not been effectively utilized, and at the same time solve the problem of lack of a method for preparing high F-value fish collagen peptides in the prior art, and provides a method for preparing high F-value collagen by hydrolyzing anglerfish skin peptide method

Method used

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  • Method of preparing high F-value collagen peptide by hydrolyzing anglerfish fishskins

Examples

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Embodiment 1

[0030] Take 1000 g of washed anglerfish skin and cut it into pieces with a length and width of about 2 cm×2 cm with scissors. Add 25L of 4.5% NaOH solution to the anglerfish skin fragments, soak for 24 h, take out, and then wash with water until the pH is neutral. Add 35 L of 9% n-butanol solution to the fish skin, stir for 28 h, take it out, wash with water and drain. Crush the drained anglerfish skin fragments, add citric acid solution with a mass fraction of 4% according to the ratio of fish skin and citric acid solution to 1:4, heat to 55°C, and continuously stir to extract After 6 h, the crude collagen extract was obtained after filtration. Then the crude collagen extract was put into an insulating bag, and subjected to high-voltage pulse electric field treatment for 0.5h. Set the pulse time of the high-voltage pulsed electric field treatment as 1s, the pulse frequency as 30Hz, and the electric field intensity as 25kV / cm. After taking it out, add 1% pepsin to the crude...

Embodiment 2

[0032]Take 1000 g of washed anglerfish skin and cut it into pieces with a length and width of about 2 cm×2 cm with scissors. Add 25L of 2.5% NaOH solution to the anglerfish skin fragments, soak them for 30 h, take them out, and then wash them with water until the pH is neutral. Add 35 L of 9% n-butanol solution to the fish skin, stir for 20 h, take it out, wash with water and drain. The drained anglerfish skin fragments were mashed, and the ratio of fish skin to acetic acid solution was 1:7, then 1% acetic acid solution was added, heated to 65°C, and extracted with constant stirring for 7 h , to obtain crude collagen extract after filtration. Then the crude collagen extract was put into an insulating bag, and subjected to high-voltage pulse electric field treatment for 1 hour. Set the pulse time of the high-voltage pulsed electric field treatment as 1s, the pulse frequency as 40Hz, and the electric field intensity as 15kV / cm. After taking it out, add 2% pepsin to the crude ...

Embodiment 3

[0034] Take 1000 g of washed anglerfish skin and cut it into pieces with a length and width of about 2 cm×2 cm with scissors. Add 25L of 5.5% NaOH solution to the anglerfish skin fragments, soak for 28 hours, take it out, and then wash with water until the pH is neutral. Then add 35 L of 9% n-butanol solution to the fish skin, take it out after stirring for 25 hours, wash with water and drain. Crush the drained anglerfish skin fragments, add citric acid solution with a mass fraction of 2% according to the ratio of fish skin and citric acid solution to 1:5, heat to 90°C, and continuously stir to extract After 5h, the crude collagen extract was obtained after filtration. Then the crude collagen extract was put into an insulating bag, and subjected to high-voltage pulse electric field treatment for 0.8h. Set the pulse time of the high-voltage pulsed electric field treatment as 1s, the pulse frequency as 35Hz, and the electric field intensity as 20kV / cm. After taking it out, ad...

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Abstract

The invention discloses a method of preparing high F-value collagen peptide by hydrolyzing anglerfish fishskins. The method comprises the following steps: by taking anglerfish fishskins as a raw material, extracting collagen by acid liquor; then, fractionally hydrolyzing fishskin collagen by using stomach protease and flavourzyme under an ultrasonic condition; and finally, removing aryl and color, and after vacuum concentration, carrying out spray drying to obtain the high F-value collagen peptide. According to the method disclosed by the invention, leftovers of the anglerfish fishskins are effectively utilized, and the high F-value collagen peptide is prepared by hydrolyzing the anglerfish fishskins, so that not only is high nutritional value of collagen peptide extracted from the anglerfish fishskins utilized, but also the problem that the leftovers of the anglerfish fishskins pollute the environment and lead to waste of resources is further solved. The collagen peptide prepared by the method disclosed by the invention has high F value and small relative molecular weight and is beneficial to be absorbed by the human body. The value of the collagen peptide is higher than that of common collagen peptide.

Description

technical field [0001] The invention relates to the field of preparation of fish collagen peptides, in particular to a method for preparing collagen peptides with high F value by hydrolyzing anglerfish skin. Background technique [0002] Anglerfish is a cold-temperate bottom fish widely distributed in the eastern waters of my country (the northern part of the East China Sea, the Yellow Sea and the Bohai Sea). In recent years, with the continuous increase in the processing of anglerfish in my country, a large amount of leftovers such as fish skin are left in the processing process, which pollutes the environment and causes a waste of resources. Relevant personnel have done relevant research on chondroitin sulfate, dermatan sulfate, collagen and other active substances in the by-products of anglerfish processing. It has been found that the preparation of collagen peptides by hydrolyzing the collagen of anglerfish can significantly improve its nutritional value and digestion. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 杨会成李八方郑斌马华威
Owner OCEAN UNIV OF CHINA
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